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Mass spectrometry-based analysis of Mycobacterial single-colony proteome

ABSTRACT: Here as an attempt to explore bacterial single-colony proteomics, we describe a quantitative mass spectrometry-based protocol to isolate and analyse the proteome of a single mycobacterial colony from 7H10 media. Tryptic peptides are evaluated with high performance liquid chromatography coupled with a hybrid quadrupole mass filter Orbitrap analyser (Q Exactive) and raw data analysed using the MaxQuant Suite and downstream analysis using Perseus software . A total of 7805 unique peptides and 1387 proteins were identified

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mycobacterium Smegmatis (strain Atcc 700084 / Mc(2)155)

SUBMITTER: john Iradukunda

LAB HEAD: Jonathan Blackburn

PROVIDER: PXD018168 | Pride | 2021-03-11

REPOSITORIES: Pride

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			Action	DRS
	200216_JMI_SC_1.raw	Raw
	200217_JMI_SC_2.raw	Raw
	200217_JMI_SC_3.raw	Raw
	200217_JMI_SC_4.raw	Raw
	IdentificationresultsproducedbyMaxQuant.7z	Other

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Publications

Mass Spectrometry-Based Analysis of Mycobacterial Single-Colony Proteome.

Iradukunda John J Ganief Tariq T Blackburn Jonathan M JM Soares Nelson C NC

Methods in molecular biology (Clifton, N.J.) 20210101

Mass spectrometry-based single-cell proteomic analysis has recently gained momentum and is now an emerging area with established protocols and promising results. Traditional proteomic studies, especially involving bacteria, have been limited to suspension cultures with large protein yields. Such studies, however, remain population centered with the uniqueness of individual responses to environmental challenges becoming diluted. To enable bacterial single-colony proteomics, we describe a quantita ...[more]

PMID: 33687715

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