Dataset Information

Proteomics profiling of xanthomonas retroflexus colony variants

ABSTRACT: Plasmid conjugation is a key facilitator of horizontal gene transfer. Since plasmids often carry antibiotic resistance genes, they are crucial drivers of the world-wide rise of antibiotic resistance among pathogens. In natural, engineered and clinical environments, bacteria often grow in protective biofilms. Therefore, a better understanding of plasmid transfer in biofilms is needed. Our aim was to investigate plasmid transfer in a biofilm adapted wrinkly colony mutant of Xanthomonas retroflexus (XRw) with enhanced matrix production and reduced motility. We found that XRw biofilms had an increased uptake of the broad host-range IncP-1ϵ plasmid pKJK5 compared to the wild type. Proteomics revealed fewer flagellum associated proteins in XRw, suggesting that flagella were responsible for reducing plasmid uptake. This was confirmed by the higher plasmid uptake of non-flagellated ∆fliM mutants of X. retroflexus wild type and wrinkly mutant. Moreover, testing several flagella mutants of Pseudomonas putida suggested that the flagella effect was more general. We identified seven mechanisms with the potential to explain the flagella effect and simulated them in an individual-based model. Two mechanisms could thus be eliminated (increased distances between cells and increased lag times due to flagella). Another mechanism identified as viable in the modelling was eliminated by further experiments. Four additional proposed mechanisms have a reduced probability of plasmid transfer in common. Our findings highlight the important yet complex effects of flagella during bacterial conjugation in biofilms.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Xanthomonas Retroflexus

TISSUE(S): Biofilm

SUBMITTER: Jakob Herschend

LAB HEAD: Jakob Herschend

PROVIDER: PXD040461 | Pride | 2024-01-26

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20161129_Homepacked20161011_15cm_JakobH_A.raw	Raw
	20161129_Homepacked20161011_15cm_JakobH_B.raw	Raw
	20161129_Homepacked20161011_15cm_JakobH_C.raw	Raw
	20161129_Homepacked20161011_15cm_JakobH_D.raw	Raw
	20161129_Homepacked20161011_15cm_JakobH_E.raw	Raw

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Publications

Flagellar interference with plasmid uptake in biofilms: a joint experimental and modeling study.

Røder Henriette Lyng HL Christidi Eleni E Amador Cristina I CI Music Samra S Olesen Asmus Kalckar AK Svensson Birte B Madsen Jonas Stenløkke JS Herschend Jakob J Kreft Jan-Ulrich J-U Burmølle Mette M

Applied and environmental microbiology 20231214 1

Plasmid conjugation is a key facilitator of horizontal gene transfer (HGT), and plasmids encoding antibiotic resistance drive the increasing prevalence of antibiotic resistance. In natural, engineered, and clinical environments, bacteria often grow in protective biofilms. Therefore, a better understanding of plasmid transfer in biofilms is needed. Our aim was to investigate plasmid transfer in a biofilm-adapted wrinkly colony mutant of <i>Xanthomonas retroflexus</i> (XRw) with enhanced matrix pr ...[more]

PMID: 38095456

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