Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Brain, Neuron, Oligodendrocyte, Cerebrospinal Fluid, Astrocyte, Microglia, Microglial Cell
SUBMITTER: Stephan Mueller
LAB HEAD: Stefan F. Lichtenthaler
PROVIDER: PXD018171 | Pride | 2020-09-25
REPOSITORIES: Pride
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The EMBO journal 20200921 20
To understand how cells communicate in the nervous system, it is essential to define their secretome, which is challenging for primary cells because of large cell numbers being required. Here, we miniaturized secretome analysis by developing the "high-performance secretome protein enrichment with click sugars" (hiSPECS) method. To demonstrate its broad utility, hiSPECS was used to identify the secretory response of brain slices upon LPS-induced neuroinflammation and to establish the cell type-re ...[more]