Dataset Information

Secretome Analysis of hippocampal and cortical murine neurons

ABSTRACT: To understand how cells communicate with each other, it is essential to define the cellular secretome, a collection of proteins including soluble secreted, unconventionally secreted and proteolytically-shed proteins. Quantitative methodologies to decipher the secretome are challenging, due to the requirement of large cell numbers and abundant serum proteins that interfere with the detection of low-abundant cellular secretome proteins. Here, we have use the highe perfomance secretome-protein-enrichment-with-click-sugars method (hiSPECS) for glyco-secretome analysis. We applied this method to investigate differences of hippocampal and cortical murine neurons. Additionally, we have inhibited the Alzheimer related protease BACE1 to identify potential substrates in the secretome of hippocampal neurons.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Neuron

SUBMITTER: Stephan Mueller

LAB HEAD: Stefan F. Lichtenthaler

PROVIDER: PXD020503 | Pride | 2020-09-25

REPOSITORIES: Pride

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Publications

An optimized quantitative proteomics method establishes the cell type-resolved mouse brain secretome.

Tüshaus Johanna J Müller Stephan A SA Kataka Evans Sioma ES Zaucha Jan J Sebastian Monasor Laura L Su Minhui M Güner Gökhan G Jocher Georg G Tahirovic Sabina S Frishman Dmitrij D Simons Mikael M Lichtenthaler Stefan F SF

The EMBO journal 20200921 20

To understand how cells communicate in the nervous system, it is essential to define their secretome, which is challenging for primary cells because of large cell numbers being required. Here, we miniaturized secretome analysis by developing the "high-performance secretome protein enrichment with click sugars" (hiSPECS) method. To demonstrate its broad utility, hiSPECS was used to identify the secretory response of brain slices upon LPS-induced neuroinflammation and to establish the cell type-re ...[more]

PMID: 32954517

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			Action	DRS
	C3Hip.zip	Other
	Hipvsctx.zip	Other
	iSPECS_DIA_N_Ctx_1.raw	Raw
	iSPECS_DIA_N_Ctx_2.raw	Raw
	iSPECS_DIA_N_Ctx_3.raw	Raw