Proteomics

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Stoichiometry of two plant glycine decarboxylase complexes and comparison with a cyanobacterial glycine cleavage system


ABSTRACT: The multienzyme glycine cleavage system (GCS) converts glycine and tetrahydrofolate to the one-carbon compound 5,10-methylenetetrahydrofolate, which is of vital importance for most if not all organisms. Photorespiring plant mitochondria contain very high levels of GCS proteins organized as a fragile glycine decarboxylase complex (GDC). The aim of this study is to provide mass spectrometry-based stoichiometric data for the plant leaf GDC and examine whether complex formation could be a general property of the GCS in photosynthesizing organisms. To this end, we investigated the mitochondrial content, the stoichiometry and the isoprotein composition of the Arabidopsis thaliana leaf GDC in comparison with that of Pisum sativum using stable-isotope-labeled peptides (QconCAT and synthetic labeled peptides) and compared the results to label-free, Hi3 peptide quantification.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Pisum Sativum (garden Pea) Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Photosynthetic Cell, Leaf

SUBMITTER: Stefan Mikkat  

LAB HEAD: Hermann Bauwe

PROVIDER: PXD018211 | Pride | 2020-04-23

REPOSITORIES: Pride

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Publications

Stoichiometry of two plant glycine decarboxylase complexes and comparison with a cyanobacterial glycine cleavage system.

Wittmiß Maria M   Mikkat Stefan S   Hagemann Martin M   Bauwe Hermann H  

The Plant journal : for cell and molecular biology 20200512 2


The multienzyme glycine cleavage system (GCS) converts glycine and tetrahydrofolate to the one-carbon compound 5,10-methylenetetrahydrofolate, which is of vital importance for most if not all organisms. Photorespiring plant mitochondria contain very high levels of GCS proteins organised as a fragile glycine decarboxylase complex (GDC). The aim of this study is to provide mass spectrometry-based stoichiometric data for the plant leaf GDC and examine whether complex formation could be a general pr  ...[more]

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