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Salinity adaptation in the principal arboviral vector Aedes aegypti characterized by larval cuticle changes

ABSTRACT: Aedes aegypti, the principle global vector of arboviral diseases, has been widely regarded to only lay eggs and undergo preimaginal development in fresh water collections. Recent observations however show that it has adapted to develop in anthropogenic brackish water habitats of up to 50% sea water in coastal areas in different continents. This adaptation is characterised by greater salinity tolerance in adult oviposition preference, larvae and changes in sizes of anal papillae. The physiological basis for salinity tolerance in either Ae. aegypti or any of the known salinity-tolerant species of Anopheles malaria vectors is not established. To address this knowledge gap which is of fundamental biological interest and important for control of major diseases we performed RNAseq analysis of gut, anal papillae, and rest of the carcass of Ae. aegypti collected in the field from brackish water (BW) and fresh water habitats (FW) and then maintained as laboratory colonies in BW and FW respectively. We also examined the cuticle structure of larvae, pupae and adult BW and FW Ae. aegypti by microscopy and performed proteomic analysis of the shed cuticles of fourth instar larvae (L4) when they transformed into pupae. The results show that major changes in cuticle structure and composition characterize, and may be the principal factor that permits, the adaptation of Ae. aegypti to brackish water.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Aedes Aegypti

TISSUE(S): Cuticle

SUBMITTER: Sebastien Voisin

LAB HEAD: Ranjan Ramasamy

PROVIDER: PXD018397 | Pride | 2021-04-07

REPOSITORIES: Pride

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			Action	DRS
	ESVO_NSI_A587.mzML	Mzml
	ESVO_NSI_A587.mzid.gz	Mzid
	ESVO_NSI_A587.pride.mgf.gz	Mgf
	ESVO_NSI_A587.pride.mztab.gz	Mztab
	ESVO_NSI_A587.raw	Raw

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