Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Malassezia sympodialis host response studied with an ex-vivo human skin model

ABSTRACT: An ex-vivo human skin model was used to investigate the host skin response to M. sympodialis on oily (supplemented with oleic acid) and non-oily skin. Host-pathogen interactions were analysed by different molecular techniques including proteomics.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skin

SUBMITTER: David Stead

LAB HEAD: Professor Carol A Munro

PROVIDER: PXD018404 | Pride | 2021-09-09

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	MI_OA_d6_1_human.msf	Msf
	MI_OA_d6_1_human.pep.xml	Pepxml
	MI_OA_d6_1_malassezia.msf	Msf
	MI_OA_d6_1_malassezia.pep.xml	Pepxml
	MI_OA_d6_2_human.msf	Msf

Items per page:

1 - 5 of 78

Publications

Host Responses in an Ex Vivo Human Skin Model Challenged With Malassezia sympodialis.

Corzo-León Dora E DE MacCallum Donna M DM Munro Carol A CA

Frontiers in cellular and infection microbiology 20210121

Malassezia species are a major part of the normal mycobiota and colonize mainly sebum-rich skin regions of the body. This group of fungi cause a variety of infections such as pityriasis versicolor, folliculitis, and fungaemia. In particular, Malassezia sympodialis and its allergens have been associated with non-infective inflammatory diseases such as seborrheic dermatitis and atopic eczema. The aim of this study was to investigate the host response to M. sympodialis on oily ...[more]

PMID: 33552997

Similar Datasets

Epitope shaving and fungal immune evasion – culture supernatants

Project description:The cell wall provides a major physical interface between fungal pathogens and their mammalian host. This extracellular armour is critical for fungal cell homeostasis and survival. Yet essential cell wall moieties, such as β-1,3-glucan, are recognised as pathogen-associated molecular patterns (PAMPs) that activate immune-mediated clearance mechanisms. We have reported that the opportunistic human fungal pathogen, Candida albicans, masks β-1,3-glucan following exposure to lactate, hypoxia or iron depletion. However, the precise mechanism(s) by which C. albicans masks β-1,3-glucan have remained obscure. Here, we performed proteomic analysis of supernatants harvested from C. albicans cells grown in hypoxia or lactate compared to glucose-grown controls to identify mechanisms driving β-1,3-glucan masking.

2020-06-15 | PXD018044 | Pride

Epitope shaving and fungal immune evasion – cell wall proteomes

Project description:The cell wall provides a major physical interface between fungal pathogens and their mammalian host. This extracellular armour is critical for fungal cell homeostasis and survival. Yet essential cell wall moieties, such as β-1,3-glucan, are recognised as pathogen-associated molecular patterns (PAMPs) that activate immune-mediated clearance mechanisms. We have reported that the opportunistic human fungal pathogen, Candida albicans, masks β-1,3-glucan following exposure to lactate, hypoxia or iron depletion. However, the precise mechanism(s) by which C. albicans masks β-1,3-glucan have remained obscure. Here, we performed proteomic analysis of cell walls from C. albicans cells grown in hypoxia or lactate compared to glucose-grown controls to identify mechanisms driving β-1,3-glucan masking.

2020-06-15 | PXD018027 | Pride

Trancriptomic analysis in Salmonella typhimurium infection [invA_vs_14028s]

Project description:au10-06_salmonella_eduardob - inva_vs_14028s Transcriptomic analysis between Salmonella wt and different mutants in type3 secretion system. - Plants were grown during 14 days in solid medium and were transfered to liquid medium during 16 h. at 9 am samonella was added to a final concentration of OD600=0.2 2 dye-swap - normal vs disease comparison

2012-01-31 | E-GEOD-34664 | biostudies-arrayexpress

plant rabt nÂ°1-Plant RABT nÂ°1

Project description:adt12-01_uprt - plant rabt nÂ°1 - Impact of RNA labeling by Plant RABT on plant transcriptome - Determine the incidence of RNA marking by the RABT method Plantation on the plants transcriptome. In several studies Clearly et al. have described a method referred as "4TU tagging" which can be use to study mRNA synthesis and decay either in a mixed population of cells or in a specific cell type (Cleary, Meiering et al. 2005, Zeiner, Cleary et al. 2008, Miller, Robinson et al. 2009, Rabani et al., 2011). Through the specific cell type expression in drosophila and mammalian cells of theToxoplasma gondii uracil PhosphoribosylTransferase activity and the metabolization of the uracil analog 4-thiouracil (4 TU), mRNA were selectively tagged, purified and used in microarray based analysis. 6 dye-swap - treated vs untreated comparison

2014-09-10 | E-GEOD-60532 | biostudies-arrayexpress

gravity-Gravitational signature of synchronized cell cultures in particular cell cycle stages

Project description:au13-11_gravity - gravity - Cell cycle and cell proliferation are decoupled under altered gravity conditions. We have previously shown that semisolid cell cultures of Arabidopsis suffer overall genome changes in response to altered gravity and also that cell cycle stages duration is altered. By using synchronized cell cultures we will demonstrate the precise alterations in cell cycle duration and also the transcriptional signature in any of them. - Experiments consists on exposures of Arabidopsis cell cultures to 1g control/simulated microgravity (RPM) conditions. Asynchronous cells exposed for 14 h + Syncronous populations choosen to have an enrichment of cell cycle phases were used (being T7/T10 samples on G2 phase, T14/T16 samples on G1 phase). 6 dye-swap - time course,treated vs untreated comparison

2014-08-23 | E-GEOD-60473 | biostudies-arrayexpress

cdt1_bis; Transcriptome comparison of the crl mutant and CDT1-RNAi line

Project description:au13-04_cdtbis; cdt1_bis crl mutants and CDT1-RNAi lines have very similar macroscopic phenotypes as well as identical defects in plastid division and biogenesis. Our goal wwas to determine how much of these similarities originated from similar alterations of gene expression. Plantlets of crl mutant and CDT1-RNAi lines were grown in vitro on MS1/2 medium for 14 days. CDT1-RNAi lines were compared to the corresponding wild-type (Ws), whereas crl mutants were compared to their wild-type siblings and are in the Col0 ecotype. 4 dye-swap - normal vs rnai mutant comparaison, normal vs transgenic comparaison

2013-09-01 | E-GEOD-48465 | biostudies-arrayexpress

Influence of Nod Factors on early developmental stages of root development in Medicago truncatula

Project description:We would like to know how symbiotic molecules such as Nod Factors (NF) influence lateral root (LR) development in M. truncatula. We have preliminary evidence that this action is through early stages of root development. Auxin is the major phytohormone controlling LR development and we also have evidence that NF interfere with auxin for the control of LR development. This transcriptomic study aims at finding new molecular targets that would be responsive to auxin and NF treatment, even at a higher level by the combination of both auxin and NF. Such targets could be involved in the control or early development stages of LR that would be controlled both by auxin and NF treatment. - 2 day old M. truncatula (accession A17) plantlets grown on M medium were transferred for 2 days on M medium+ 10-5M NPA (1-N-Naphthylphthalamic acid, auxin transport inhibitor) then transferred for 10 h on M medium containing 10-6 M NAA (naphthalene-1-acetic acid, permeant auxin analog) or 10-7 M Nod factors (NF) or a combination of both (10-6 M NAA + 10-7 M NF). These are compared to mock treated (solvent only) plants. 12plex_med_2013_04 12 dye-swap - treated vs untreated comparison

2016-06-01 | E-GEOD-74099 | biostudies-arrayexpress

myb118 mutation; Identification of genes misregulated in developing seeds of Arabidopsis in response to the myb118 mutation.

Project description:ra12-06_myb118; myb118 mutation What are the genes over expressed or down regulated in seeds in response to a myb118 mutation? Plants of the different genotypes (wild-type, myb118 mutant, myb118 antisense lines named 0E3 and T16) were grown in a green house, their flower were tagged and seeds were harvested 10 days after anthesis. Seeds were extracted from the siliques, stored at -80Â°C prior to RNA extraction. 6 dye-swap - normal vs transgenic comparaison

2013-12-01 | E-GEOD-48180 | biostudies-arrayexpress

cpsf30 mips1 transcriptome-Cell death control by CPSF30

Project description:au12-06_cpsf30 - cpsf30 mips1 transcriptome - How a mutation in the CPSF30 is able to suppress the cell death phenotype observed in the mips1-1 Arabidopsis mutant? - All the plants were cultivated in the same hanging conditions 10days in "short Days" then in long Days. The takings were later made 4days in "long Days" what corresponds to the appearance of the symptoms of cellular death at the mutant mips1-1. On the other hand, the gene CPSF30 codes for two transcribed. We have a double mutant mips1 cpsf30 complemented with the transcribed young. 6 dye-swap - genotype comparaison

2014-02-01 | E-GEOD-53247 | biostudies-arrayexpress

abaseed-ABA signaling in Arabidopsis seeds

Project description:ra14-04_nced - abaseed - Identification of abscisic acid (ABA) signaling factors and targets in Arabidopsis developing seeds by comparing ABA deficient and wild type genotypes. Transcriptome comparison of developing seeds harvested at 14 DAP from two ABA-deficient mutants (nced2 nced5 nced9 and nced2 nced5 nced6 nced9) and wild type (Columbia-0). 6 dye-swaps - gene knockout, genomic comparison.

2015-07-01 | E-GEOD-68088 | biostudies-arrayexpress