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Thermal stability variation during transtion to pluripotency

ABSTRACT: To investigate functional differences between pluripotent stem cells and somatic cells, we measured protein thermal stability and expression after transitions between cell types using allogenic cell lines. To this purpose, we reprogrammed hFF into human iPSCs and initiated undirected differentiation through formation of EBs during 21 days. We also included human embryonic stem cells (ESC) in order to validate our approach and colon cancer cell line RKO.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pluripotent Stem Cell, Fibroblast, Embryonic Stem Cell

SUBMITTER: Pierre Sabatier

LAB HEAD: Roman A Zubarev

PROVIDER: PXD018453 | Pride | 2021-10-05

REPOSITORIES: Pride

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			Action	DRS
	20200226_PISA_PSC_1-1.raw	Raw
	20200226_PISA_PSC_1-10.raw	Raw
	20200226_PISA_PSC_1-11.raw	Raw
	20200226_PISA_PSC_1-12.raw	Raw
	20200226_PISA_PSC_1-13.raw	Raw

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SBDS-dependent ribosome destabilization maintains pluripotency via regulation of translation

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