Dataset Information

Identification of the Cfap206 interactome from mouse testis to identify its role in cilia function

ABSTRACT: Cfap206 was immunoprecipitated from mouse testis to identify the interactome of this protein for further functional studies.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis, Sperm

DISEASE(S): Ciliopathy

SUBMITTER: Karsten Boldt

LAB HEAD: Dr. Karsten Boldt

PROVIDER: PXD018554 | Pride | 2020-05-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20190410_Cfap206.zip	Other
	JUN2917-S06-A-KO2_2_Eluat1.raw	Raw
	JUN2917-S07-A-WT2_1_Eluat1.raw	Raw
	JUN2917-S08-A-KO2_3_Eluat1.raw	Raw
	JUN2917-S09-A-WT2_2_Eluat1.raw	Raw

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Publications

The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development.

Beckers Anja A Beckers Anja A Adis Christian C Schuster-Gossler Karin K Tveriakhina Lena L Ott Tim T Fuhl Franziska F Hegermann Jan J Boldt Karsten K Serth Katrin K Rachev Ev E Alten Leonie L Kremmer Elisabeth E Ueffing Marius M Blum Martin M Gossler Achim A

Development (Cambridge, England) 20200615 21

Cilia are complex cellular protrusions consisting of hundreds of proteins. Defects in ciliary structure and function, many of which have not been characterised molecularly, cause ciliopathies: a heterogeneous group of human syndromes. Here, we report on the FOXJ1 target gene Cfap206, orthologues of which so far have only been studied in Chlamydomonas and Tetrahymena In mouse and Xenopus, Cfap206 was co-expressed with and dependent on Foxj1 CFAP206 protei ...[more]

PMID: 32376681

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Project description:The plant pathogenic fungus Verticillium dahliae has homologs of the clock genes [Cascant-Lopez et al., 2020], which are well described in Neurospora crassa [Diernfellner et al., 2020]. In N. crassa, frequency (Frq) and the Frq-interacting RNA helicase (Frh) interact and form the Frq-Frh complex [Cheng et al., 2005]. Formation of this complex was described to be still possible but weakened by a single amino acid exchange of arginine 806 of Frh to histidine (Frh^R806H) [Shi et al., 2010; Conrad et al., 2016]. We found that both FRQ and wild-type FRH were required for enhanced conidiation and the light-dependent repression of microsclerotia formation in V. dahliae, but were dispensable for disease induction in tomato plants. Strikingly, the FRH^R806H mutant strain grew similar to the FRQ deletion strain, although transcript and protein levels of Frq were not significantly affected by the FRH^R806H mutation when compared with the wild-type. Therefore, we hypothesized that V. dahliae Frq and Frh form the Frq-Frh complex to regulate V. dahliae development, and that this complex formation is abolished upon the amino acid exchange in the Frh. By the use of protein pull-downs, potential interactions of Frh-GFP or Frh^R806H-GFP with Frq were investigated. While we found evidence for a direct interaction of Frh-GFP and Frq, no proteins were significantly co-enriched with Frh^R806H-GFP. References: Cascant-Lopez, E.; Crosthwaite, S.K.; Johnson, L.J.; Harrison, R.J. No evidence that homologs of key circadian clock genes direct circadian programs of development or mRNA abundance in Verticillium dahliae. Front Microbiol 2020, 11, 1977, doi:10.3389/fmicb.2020.01977. Diernfellner, A.C.R.; Brunner, M. Phosphorylation timers in the Neurospora crassa circadian clock. J Mol Biol 2020, 432, 3449–3465, doi:10.1016/j.jmb.2020.04.004. Cheng, P.; He, Q.; He, Q.; Wang, L.; Liu, Y. Regulation of the Neurospora circadian clock by an RNA helicase. Genes Dev 2005, 19, 234–241, doi:10.1101/gad.1266805. Shi, M.; Collett, M.; Loros, J.J.; Dunlap, J.C. FRQ-interacting RNA helicase mediates negative and positive feedback in the Neurospora circadian clock. Genetics 2010, 184, 351–361, doi:10.1534/genetics.109.111393. Conrad, K.S.; Hurley, J.M.; Widom, J.; Ringelberg, C.S.; Loros, J.J.; Dunlap, J.C.; Crane, B.R. Structure of the frequency‐interacting RNA helicase: a protein interaction hub for the circadian clock. EMBO J 2016, 35, 1707–1719, doi:10.15252/embj.201694327.

Dataset Information

Identification of the Cfap206 interactome from mouse testis to identify its role in cilia function

Dataset's files

Publications

The FOXJ1 target <i>Cfap206</i> is required for sperm motility, mucociliary clearance of the airways and brain development.

Similar Datasets

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