Dataset Information

VE-cadherin associated proteins in the absence of lncRNA LASSIE

ABSTRACT: The shear stress-regulated lncRNA LASSIE interacts with junctional proteins (e.g. PECAM-1, which interacts with VE-cadherin) and influences endothelial barrier function. Here we characterize the remodeling of the VE-Cadherin complex by the lncRNA LASSIE. LASSIE silenced HUVECs were subjected to co-immunoprecipitation using an anti-VE-cadherin antibody. Differentially associated proteins were identified by Mass spectrometry. This analysis revealed a significantly decreased association of cytoskeleton-linked proteins with VE-cadherin after silencing of LASSIE. Functional assays confirmed this result and characterized LASSIE as a stabilizer of junctional complexes in endothelial cells, important for normal shear stress sensing and barrier function.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Ilka Wittig

LAB HEAD: Reinier Boon

PROVIDER: PXD018725 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Data_analysis.xlsx	Xlsx
	P18_019_Stanicek_IP_Sample01_B.raw	Raw
	P18_019_Stanicek_IP_Sample01_C.raw	Raw
	P18_019_Stanicek_IP_Sample01_D.raw	Raw
	P18_019_Stanicek_IP_Sample01_E.raw	Raw

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Publications

Long non-coding RNA LASSIE regulates shear stress sensing and endothelial barrier function.

Stanicek Laura L Lozano-Vidal Noelia N Bink Diewertje Ilse DI Hooglugt Aukie A Yao Wenjie W Wittig Ilka I van Rijssel Jos J van Buul Jaap Diederik JD van Bergen Anke A Klems Alina A Ramms Anne Sophie AS Le Noble Ferdinand F Hofmann Patrick P Szulcek Robert R Wang ShengPeng S Offermanns Stefan S Ercanoglu Meryem Seda MS Kwon Hyouk-Bum HB Stainier Didier D Huveneers Stephan S Kurian Leo L Dimmeler Stefanie S Boon Reinier Abraham RA

Communications biology 20200526 1

Blood vessels are constantly exposed to shear stress, a biomechanical force generated by blood flow. Normal shear stress sensing and barrier function are crucial for vascular homeostasis and are controlled by adherens junctions (AJs). Here we show that AJs are stabilized by the shear stress-induced long non-coding RNA LASSIE (linc00520). Silencing of LASSIE in endothelial cells impairs cell survival, cell-cell contacts and cell alignment in the direction of flow. LASSIE associates with junction ...[more]

PMID: 32457386

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