Proteomics

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Identification of protein interaction partners of the lncRNA Lassie

ABSTRACT: The shear stress-regulated lncRNA LASSIE interferes with endothelial cell function as knockdown of Lassie affects apoptosis, proliferation and angiogenic sprouting in vitro. RNA-antisense purification and subsequent mass spectrometry analysis identifed junctional proteins (such as PECAM-1) as interactors of LASSIE . Additional functional analyses demonstrate a role of LASSIE in shear stress sensing and barrier function in endothelial cells, by stabilizing endothelial cell junctions through connection to the cytoskeleton.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Ilka Wittig  

LAB HEAD: Reinier Boon

PROVIDER: PXD018724 | Pride | 2021-07-22

REPOSITORIES: Pride

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Data_analysis.xlsx Xlsx
P17_022_DZHK_LS_Lassie_E_01.raw Raw
P17_022_DZHK_LS_Lassie_E_02.raw Raw
P17_022_DZHK_LS_Lassie_E_03.raw Raw
P17_022_DZHK_LS_Lassie_E_04.raw Raw
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Publications

Blood vessels are constantly exposed to shear stress, a biomechanical force generated by blood flow. Normal shear stress sensing and barrier function are crucial for vascular homeostasis and are controlled by adherens junctions (AJs). Here we show that AJs are stabilized by the shear stress-induced long non-coding RNA LASSIE (linc00520). Silencing of LASSIE in endothelial cells impairs cell survival, cell-cell contacts and cell alignment in the direction of flow. LASSIE associates with junction  ...[more]

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