Proteomics

Dataset Information

0

Thermal proteome profiling of Toxoplasma gondii lysates treated with enhancer 1 (ENH1)

ABSTRACT: Previously, we characterized a chemical modulator, ENH1, that exhibited potent antiparasitic properties against apicomplexans and induced calcium oscillations, but the target eluded identification. To determine the target of ENH1, we employed thermal proteome profiling in parasites. We treated extracellular parasites with vehicle (DMSO) or ENH1 for 15 minutes at 37°C, under conditions previously determined to induce calcium fluxes in T. gondii.8 Parasites were then heated at 10 different temperatures to induce thermal denaturation. Following lysis and high-speed centrifugation, soluble protein was isolated and analyzed by mass spectrometry to generate thermal stability profiles from each condition across two biological replicates.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Toxoplasma Gondii Gt1

SUBMITTER: Alice Herneisen  

LAB HEAD: Sebastian Lourido

PROVIDER: PXD018810 | Pride | 2020-07-13

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20200106_CDPK1M_ENH1_LCETSA_DMSO_1.raw Raw
20200106_CDPK1M_ENH1_LCETSA_DMSO_2.raw Raw
20200106_CDPK1M_ENH1_LCETSA_DMSO_3.raw Raw
20200106_CDPK1M_ENH1_LCETSA_DMSO_4.raw Raw
20200106_CDPK1M_ENH1_LCETSA_DMSO_5.raw Raw
Items per page:
1 - 5 of 24
altmetric image

Publications

Identifying the Target of an Antiparasitic Compound in <i>Toxoplasma</i> Using Thermal Proteome Profiling.

Herneisen Alice L AL   Sidik Saima M SM   Markus Benedikt M BM   Drewry David H DH   Zuercher William J WJ   Lourido Sebastian S  

ACS chemical biology 20200706 7

Apicomplexan parasites include the causative agents of malaria and toxoplasmosis. Cell-based screens in <i>Toxoplasma</i> previously identified a chemical modulator of calcium signaling (ENH1) that blocked parasite egress from host cells and exhibited potent antiparasitic activity. To identify the targets of ENH1, we adapted thermal proteome profiling to <i>Toxoplasma</i>, which revealed calcium-dependent protein kinase 1 (CDPK1) as a target. We confirmed the inhibition of CDPK1 by ENH1 in vitro  ...[more]

Similar Datasets

Proteomics Thermal proteome profiling of Toxoplasma gondii treated with enhancer 1 (ENH1)
2023-08-21 | PXD018818 | Pride
Proteomics Identification of proteins exhibiting TgDegP2-responsive thermal stability
2020-08-28 | PXD019917 | Pride
Proteomics Nuclear release of eIF1 globally increases stringency of start codon selection to preserve mitotic arrest physiology
2024-10-07 | PXD054183 | Pride
Proteomics De novo mapping of the apicomplexan Ca2+-responsive proteome: concentration range thermal profiling experiment 2
2022-10-15 | PXD033650 | Pride
Proteomics De novo mapping of the apicomplexan Ca2+-responsive proteome: temperature range thermal profiling experiment
2022-10-15 | PXD033713 | Pride
Proteomics De novo mapping of the apicomplexan Ca2+-responsive proteome: concentration range experiment 1
2022-10-15 | PXD033642 | Pride
Proteomics A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo development
2024-08-28 | PXD054119 | Pride
Proteomics SPARKEL depletion time course proteome
2024-07-12 | PXD040635 | Pride
Proteomics T. gondii SPARK depletion phosphoproteome
2024-07-12 | PXD040602 | Pride
Proteomics PKA C3 depletion phosphoproteomics
2024-07-12 | PXD042989 | Pride