Proteomics

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Evaluation of proteomics strategies for low cell numbers: SP3- and C18-based protocols combined with five cell lysis methods


ABSTRACT: New advances in the transcriptomics and flow cytometry fields have allowed the discovery of new immune cell subsets. However, most of them are extremely small populations, hampering their analysis using mass spectrometry strategies since currently these procedures are not highly efficient in small sample sizes. In this context, recent studies have improved sample processing for quantity-limited samples. Here, we aim at evaluating the combination of five cell lysis methods and two sample clean-up techniques (i.e. C18 and SP3) for the analysis of small cell numbers (2.500, 10.000, 50.000) of THP-1, differentiated THP-1 and HT-29 cell lines.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Paula Díez  

LAB HEAD: Paula Díez, PhD

PROVIDER: PXD018872 | Pride | 2023-02-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
L20182001798a.mgf Mgf
L20182001798a.pride.mgf.gz Mgf
L20182001798b.mgf Mgf
L20182001798b.pride.mgf.gz Mgf
L20182001798c.mgf Mgf
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