Dataset Information

Phosphate and phosphite differentially impact the proteome and phosphoproteome of Arabidopsis suspension cell cultures

ABSTRACT: Phosphorus is an essential limited nutrient for plant growth and modern agricultural productivity. A comprehensive understanding of how plants respond to phosphate starvation is essential to develop more phosphate-use efficient crops. Here we quantified protein-level responses to phosphate (PO4-) and phosphite (PO3-) in Arabidopsis thaliana suspension cells, relative to a phosphate-starved state using label-free proteomics and phosphoproteomics. Phosphite is similarly sensed and taken up by plants as phosphate but cannot be used as a source of phosphorus. Phosphite is hence a useful tool to delineate between non-specific processes related to sensing and transport, and specific responses to phosphorus nutrition. We find that responses to phosphate and phosphite occur mainly at the level of protein phosphorylation, complemented by limited changes in protein abundance, primarily in protein translation, and phosphate transport and scavenging proteins. Phosphorylation changes in proteins involved in core processes such as translation, RNA splicing and kinase signalling are especially important in phosphate starvation. We also find differential phosphorylation in response to phosphate and phosphite in 69 proteins, including splicing factors, translation factors, the PHT1;4 phosphate transporter and the HAT1 histone acetyltransferase—potential phospho-switches sensing changes in phosphorus nutrition. Our study hence illuminates several new aspects of the phosphate-starvation response and identifies important targets for further investigation and crop improvement.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Richard Uhrig

LAB HEAD: Richard Uhrig

PROVIDER: PXD018989 | Pride | 2021-01-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20181214_LFQ_ATcell_07_TP_10_-P.raw	Raw
	20181214_LFQ_ATcell_08_TP_3_P.raw	Raw
	20181214_LFQ_ATcell_09_TP_7_Phi.raw	Raw
	20181214_LFQ_ATcell_10_TP_4_P.raw	Raw
	20181214_LFQ_ATcell_11_TP_5_-P.raw	Raw

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Phosphate and phosphite have a differential impact on the proteome and phosphoproteome of Arabidopsis suspension cell cultures.

Mehta Devang D Ghahremani Mina M Pérez-Fernández Maria M Tan Maryalle M Schläpfer Pascal P Plaxton William C WC Uhrig R Glen RG

The Plant journal : for cell and molecular biology 20201219 4

Phosphorus absorbed in the form of phosphate (H2 PO4- ) is an essential but limiting macronutrient for plant growth and agricultural productivity. A comprehensive understanding of how plants respond to phosphate starvation is essential for the development of more phosphate-efficient crops. Here we employed label-free proteomics and phosphoproteomics to quantify protein-level responses to 48 h of phosphate versus phosphite (H2 PO3- ...[more]

PMID: 33184936

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