Proteomics

Dataset Information

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γ-glutamylcysteine (GGC) supplementation and p-LPS challenge causes distinct proteomic changes to primary airway epithelial cell models of Cystic Fibrosis


ABSTRACT: Cystic fibrosis (CF) is a life limiting inherited condition associated with recurrent bacterial infections, inflammation, oxidative stress and loss of lung function. CF is characterised by deficiencies in both intra- and extracellular glutathione (GSH) levels. This GSH depletion is exacerbated by bacterial infections which further contributes to cellular oxidative stress and the inadequate control of inflammatory pathways in CF patients. A considerable body of research supports targeting the GSH biosynthesis pathway as a therapeutic strategy, however, current therapies have not demonstrated relevant improvements in CF clinical outcomes. It has previously shown that the immediate pre-cursor to glutathione, γ-glutamylcysteine (GGC) is effective in increasing intracellular levels of GSH in vivo in healthy humans. In this in-vitro primary cell line study, we study the proteomic changes involved in treating airway epithelial cells with GGC both prior to and post-LPS challenge, and without LPS challenge.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bronchial Epithelial Cell, Epithelial Cell

DISEASE(S): Cystic Fibrosis

SUBMITTER: Alexander Capraro  

LAB HEAD: Shafagh Waters

PROVIDER: PXD019084 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Novel Antioxidant Therapy with the Immediate Precursor to Glutathione, γ-Glutamylcysteine (GGC), Ameliorates LPS-Induced Cellular Stress in In Vitro 3D-Differentiated Airway Model from Primary Cystic Fibrosis Human Bronchial Cells.

Hewson Chris K CK   Capraro Alexander A   Wong Sharon L SL   Pandzic Elvis E   Zhong Ling L   Fernando Bentotage S M BSM   Awatade Nikhil T NT   Hart-Smith Gene G   Whan Renee M RM   Thomas Shane R SR   Jaffe Adam A   Bridge Wallace J WJ   Waters Shafagh A SA  

Antioxidants (Basel, Switzerland) 20201130 12


Systemic glutathione deficiency, inflammation, and oxidative stress are hallmarks of cystic fibrosis (CF), an inherited disease that causes persistent lung infections and severe damage to the respiratory system and many of the body organs. Improvements to current antioxidant therapeutic strategies are needed. The dietary supplement, γ-glutamylcysteine (GGC), which is the immediate precursor to glutathione, rapidly boosts cellular glutathione levels following a single dose in healthy individuals.  ...[more]

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