Dataset Information

Ablation of the miRNA cluster 24 has profound effects on extracellular matrix protein abundance in cartilage

ABSTRACT: MicroRNAs (miRNAs) regulate cartilage differentiation and contribute to the onset and progression of joint degeneration. These small RNA molecules may affect extracellular matrix organization (ECM) in cartilage, but for only a few miRNAs has this role been defined in vivo. Previously, we showed that cartilage-specific genetic ablation of the Mirc24 cluster in mice leads to impaired cartilage development due to increased RAF/MEK/ERK pathway activation. Here, we studied the expression of the cluster in cartilage by LacZ reporter gene assays and determined its role for extracellular matrix homeostasis by proteome and immunoblot analysis. The cluster is expressed in prehypertrophic/hypertrophic chondrocytes of the growth plate and we now show that the cluster is also highly expressed in articular cartilage. Cartilage-specific loss of the cluster leads to increased proteoglycan 4 and matrix matrix metallopeptidase 13 levels and decreased aggrecan and collagen X levels in epiphyseal cartilage. Interestingly, these changes are linked to a decrease in SRY-related HMG box-containing (SOX) transcription factors 6 and 9, which regulate ECM production in chondrocytes. Our data suggests that the Mirc24 cluster is important for ECM homoeostasis and the expression of transcriptional regulators of matrix production in cartilage.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cartilage

SUBMITTER: Veronika S. Georgieva

LAB HEAD: Bent Brachvogel

PROVIDER: PXD019374 | Pride | 2020-06-18

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20191225_Q2_UC_ColID_952_ProjID_1989_Brachtvogel_Sample_1.raw	Raw
	20191225_Q2_UC_ColID_952_ProjID_1989_Brachtvogel_Sample_10.raw	Raw
	20191225_Q2_UC_ColID_952_ProjID_1989_Brachtvogel_Sample_2.raw	Raw
	20191225_Q2_UC_ColID_952_ProjID_1989_Brachtvogel_Sample_3.raw	Raw
	20191225_Q2_UC_ColID_952_ProjID_1989_Brachtvogel_Sample_4.raw	Raw

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Publications

Ablation of the miRNA Cluster 24 Has Profound Effects on Extracellular Matrix Protein Abundance in Cartilage.

Georgieva Veronika S VS Etich Julia J Bluhm Björn B Zhu Mengjie M Frie Christian C Wilson Richard R Zaucke Frank F Bateman John J Bateman John J Brachvogel Bent B

International journal of molecular sciences 20200609 11

MicroRNAs (miRNAs) regulate cartilage differentiation and contribute to the onset and progression of joint degeneration. These small RNA molecules may affect extracellular matrix organization (ECM) in cartilage, but for only a few miRNAs has this role been defined <i>in vivo</i>. Previously, we showed that cartilage-specific genetic ablation of the Mirc24 cluster in mice leads to impaired cartilage development due to increased RAF/MEK/ERK pathway activation. Here, we studied the expression of th ...[more]

PMID: 32526967

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