Dataset Information

Proteomic analysis of Pseudomonas aeruginosa purified outer membrane vesicle (OMV) extracts exposed to sex steroids

ABSTRACT: The project aims to elucidate mechanisms driving P. aeruginosa response to sex steroids hormones. An experiment involving outer membrane vesicles (OMV) extraction combined with Mass spectroscopy was performed to identify differences in P. aeruginosa OMVs proteins produced while being exposed to estradiol and testosterone.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Pseudomonas Aeruginosa Pao1

SUBMITTER: Valerie Yong

LAB HEAD: Sanjay Haresh Chotirmall

PROVIDER: PXD019470 | Pride | 2021-09-08

REPOSITORIES: Pride

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Publications

Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa.

Vidaillac Celine C Yong Valerie Fei Lee VFL Aschtgen Marie-Stephanie MS Qu Jing J Yang Shuowei S Xu Guangfu G Seng Zi Jing ZJ Brown Alexandra C AC Ali Md Khadem MK Jaggi Tavleen K TK Sankaran Jagadish J Foo Yong Hwee YH Righetti Francesco F Nedumaran Anu Maashaa AM Mac Aogáin Micheál M Roizman Dan D Richard Jean-Alexandre JA Rogers Thomas R TR Toyofuku Masanori M Luo Dahai D Loh Edmund E Wohland Thorsten T Czarny Bertrand B Horvat Jay C JC Hansbro Philip M PM Yang Liang L Li Liang L Normark Staffan S Henriques Normark Birgitta B Chotirmall Sanjay H SH

mBio 20200929 5

Estrogen, a major female sex steroid hormone, has been shown to promote the selection of mucoid <i>Pseudomonas aeruginosa</i> in the airways of patients with chronic respiratory diseases, including cystic fibrosis. This results in long-term persistence, poorer clinical outcomes, and limited therapeutic options. In this study, we demonstrate that at physiological concentrations, sex steroids, including testosterone and estriol, induce membrane stress responses in <i>P. aeruginosa</i> This is char ...[more]

PMID: 32994320

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Project description:Several studies indicate that adult stem cells may improve the recovery from acute tissue injury. It has been suggested that they may contribute to tissue regeneration by the release of paracrine factors promoting proliferation of tissue resident cells. However, the factors involved remain unknown. In the present study we found that microvesicles (MV) derived from human liver stem cells (HLSC) were able to stimulate in vitro proliferation and apoptosis resistance of human and rat hepatocytes. These effects required internalization of MV in the hepatocytes by an alpha4 integrin-dependent mechanism. However, when treated with RNase, MV despites their internalization were unable to induce hepatocyte proliferation and apoptosis resistance, suggesting an RNA dependent effect. Microarray analysis and quantitative RT-PCR demonstrated that MV were shuttling a specific subset of cellular mRNA, such as mRNA associated in the control of transcription, translation, proliferation and apoptosis. When administered in vivo, MV were found to accelerate the morphological and functional recovery of liver in a model of 70% hepatectomy in rats by inducing an hepatocytes proliferation that was abolished by RNase treatment. Using human AGO2 gene, which is shuttled by MV, as a reporter gene, we found the expression of human AGO2 mRNA and protein in the liver of hepatectomized rats treated with MV. This suggest a translation of the MV shuttled mRNA within hepatocytes of treated rats. Conclusion: these results suggest that MV derived from HLSC may activate a proliferative program in remnant hepatocytes after hepatectomy by a horizontal transfer of specific mRNA subsets. MV contained mRNA was submitted to microarray analysis not to define the amount of mRNA but only to define which transcripts were present. Total RNA was prepared from two independent preparation of vesicles. 250, 500 and 1000 ngs from each preparation were transformed in biotin-labeled cRNA. A simple statistical linear model was used to identify transcript signals linearly correlated to the increment of total RNA concentration used to prepare cRNA.

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