Dataset Information

Comparison of purified membrane vesicles from L. casei submitted or not to density gradient ultracentrifugation

ABSTRACT: Probiotics are defined by the World Health Organization as “live microorganisms which, when administered in adequate amounts confer a health benefit on the host”. A large number of probiotic bacteria used in the food industry are members of the diverse Lactobacillaceae family and are “Generally Regarded as Safe” (GRAS). Here, we aimed at characterizing the proteome of membrane vesicles (MVs) from Lacticasibacillus casei. We notably analyzed the proteomes of vesicles purified by density gradient ultracentrifugation (pMVs) or not (MVs).

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Lacticaseibacillus Casei Bl23

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Couté

PROVIDER: PXD036342 | Pride | 2023-01-25

REPOSITORIES: Pride

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Publications

Membrane vesicles released by Lacticaseibacillus casei BL23 inhibit the biofilm formation of Salmonella Enteritidis.

da Silva Barreira David D Laurent Julie J Lourenço Jessica J Novion Ducassou Julia J Couté Yohann Y Guzzo Jean J Rieu Aurélie A

Scientific reports 20230120 1

Biofilms represent a major concern in the food industry and healthcare. The use of probiotic bacteria and their derivatives as an alternative to conventional treatments to fight biofilm development is a promising option that has provided convincing results in the last decades. Recently, membrane vesicles (MVs) produced by probiotics have generated considerable interest due to the diversity of roles they have been associated with. However, the antimicrobial activity of probiotic MVs remains to be ...[more]

PMID: 36670157

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Project description:Several studies indicate that adult stem cells may improve the recovery from acute tissue injury. It has been suggested that they may contribute to tissue regeneration by the release of paracrine factors promoting proliferation of tissue resident cells. However, the factors involved remain unknown. In the present study we found that microvesicles (MV) derived from human liver stem cells (HLSC) were able to stimulate in vitro proliferation and apoptosis resistance of human and rat hepatocytes. These effects required internalization of MV in the hepatocytes by an alpha4 integrin-dependent mechanism. However, when treated with RNase, MV despites their internalization were unable to induce hepatocyte proliferation and apoptosis resistance, suggesting an RNA dependent effect. Microarray analysis and quantitative RT-PCR demonstrated that MV were shuttling a specific subset of cellular mRNA, such as mRNA associated in the control of transcription, translation, proliferation and apoptosis. When administered in vivo, MV were found to accelerate the morphological and functional recovery of liver in a model of 70% hepatectomy in rats by inducing an hepatocytes proliferation that was abolished by RNase treatment. Using human AGO2 gene, which is shuttled by MV, as a reporter gene, we found the expression of human AGO2 mRNA and protein in the liver of hepatectomized rats treated with MV. This suggest a translation of the MV shuttled mRNA within hepatocytes of treated rats. Conclusion: these results suggest that MV derived from HLSC may activate a proliferative program in remnant hepatocytes after hepatectomy by a horizontal transfer of specific mRNA subsets. MV contained mRNA was submitted to microarray analysis not to define the amount of mRNA but only to define which transcripts were present. Total RNA was prepared from two independent preparation of vesicles. 250, 500 and 1000 ngs from each preparation were transformed in biotin-labeled cRNA. A simple statistical linear model was used to identify transcript signals linearly correlated to the increment of total RNA concentration used to prepare cRNA.

			Action	DRS
	Identifications.mzid.gz	Mzid
	MVs_Band1_R1.mgf	Mgf
	MVs_Band1_R1.raw	Raw
	MVs_Band1_R2.mgf	Mgf
	MVs_Band1_R2.raw	Raw