Proteomics

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Human corneal epithelial cells internalize Aspergillus flavus spores by actin mediated endocytosis


ABSTRACT: Mycotic leratitis is the corneal inflammation predominantly caused by Fusarium and Aspergillus species. Corneal epithelium is the earliest cell type encounter the invading pathogen. The innate immune responses of human corneal epithelial cells against Aspergillus flavus is not known. Here we studied the role of human corneal epithelial cells against Aspergillus flavus infection. The results showed that corneal epithelial cells internalized Aspergillus flavus conidia through actin mediated polymerization surrounding the conidia. Further the actin inhibitor cytochalasin D treatment reduced the formation actin ring around the conidia. The engulfed conidia acquired endosomal proteins as revealed by immunofluorescence analsyisis. Mass spectromtery of phagosomal proteins confirmed the recruitment of endosomal proteins and other proteins involved in phagocytosis. These results show the involvement of corneal epithelial cells in anti fungal defense.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Divya Arunachalam  

LAB HEAD: Prof. K. Dharmalingam

PROVIDER: PXD019512 | Pride | 2021-04-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AD_HCE_1123_P1_B1_1.msf Msf
AD_HCE_1123_P1_B1_1.raw Raw
AD_HCE_1123_P1_B1_2.msf Msf
AD_HCE_1123_P1_B1_2.raw Raw
AD_HCE_1123_P1_B2_1.msf Msf
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Publications

Human Corneal Epithelial Cells Internalize Aspergillus flavus Spores by Actin-Mediated Endocytosis.

Arunachalam Divya D   Namperumalsamy Venkatesh Prajna VP   Prajna Lalitha L   Kuppamuthu Dharmalingam D  

Infection and immunity 20210517 6


Human corneal epithelial (HCE) cells play a significant role in the innate immune response by secreting cytokines and antimicrobial peptides when they encounter fungal pathogens. But the detailed mechanism of attachment and engulfment of the fungal conidia by HCE cells is not well understood. Here, we show the phagocytosis of <i>Aspergillus flavus</i> conidia by RCB2280 cells and primary HCE cultures using confocal microscopy and proteomic analysis of conidium-containing phagosomes. Phalloidin s  ...[more]

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