Proteomics

Dataset Information

0

PP2A-Rts1 antagonizes Rck2-mediated hyperosmotic stress signaling in yeast


ABSTRACT: In Saccharomyces cerevisiae impairment of protein phosphatase PP2A-Rts1 leads to temperature and hyperosmotic stress sensitivity, yet the underlying mechanism and the scope of action of the phosphatase in the stress response remain elusive. Using quantitative mass spectrometry-based approaches we have identified a set of putative substrate proteins that show both, hyperosmotic stress- and PP2A-Rts1-dependent changes in their phosphorylation pattern. A comparative analysis with published MS-shotgun data revealed that the phosphorylation status of many of these sites is regulated by the MAPKAP kinase Rck2, suggesting a node of regulation. Detailed gel mobility shift assays and protein-protein interaction analysis strongly suggest that Rck2 activity is directly regulated by PP2A-Rts1 via a SLiM B56-family interaction motif, uncovering a previously unknown mechanism of how PP2A influences the response to hyperosmotic stress in Yeast.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: David Hollenstein  

LAB HEAD: Wolfgang Reiter

PROVIDER: PXD019647 | Pride | 2022-05-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
100813V_WR_wt_rts1_2chCID.raw Raw
100813V_WR_wt_rts1_DDDT.raw Raw
101020V_WR_SILAC_rts1_LysC_CID.raw Raw
101020V_WR_SILAC_rts1_LysC_DDDT.raw Raw
101029V_WR_rts1_LysC_100929.raw Raw
Items per page:
1 - 5 of 43

Similar Datasets

2021-11-02 | PXD019646 | Pride
2019-06-11 | PXD011935 | Pride
2021-03-09 | PXD016623 | Pride
2021-05-05 | PXD020395 | Pride
2013-11-01 | E-GEOD-41041 | biostudies-arrayexpress
2023-08-10 | PXD041032 | Pride
2015-03-31 | E-GEOD-56325 | biostudies-arrayexpress
2015-03-31 | E-GEOD-56324 | biostudies-arrayexpress
2019-11-12 | PXD009398 | Pride
2012-03-30 | E-MTAB-592 | biostudies-arrayexpress