Dataset Information

Endosulfines Igo1 and Igo2 promote hyperosmotic stress signaling via inhibition of protein phosphatase 2A

ABSTRACT: The hyperosmotic stress response in budding yeast is a paradigm for cellular responses to physicochemical stimuli that is often used for modeling signal transduction pathways. Here, we describe the phosphatase PP2A-Cdc55 as a novel master regulator of hyperosmotic stress signaling. We show that its inhibition by the Greatwall kinase-Endosulfine signaling module at the onset of hyperosmotic stress is crucial for cellular survival with far-reaching consequences for the stress-regulated phospho-proteome. Indeed, this mechanism is required and sufficient to induce stress-specific phosphorylation patterns. This system operates in parallel and independently of the well-established Hog1 MAP kinase pathway, affecting up to 50% of the stress-induced S/T-P motifs. Many of these motifs appear to be direct substrates of PP2A-Cdc55. We exemplify the functional impact of stress-induced inhibition of PP2A-Cdc55 on the transcriptional regulation of stress-associated genes via the transcriptional regulators Rph1 and Gis1.

INSTRUMENT(S): Orbitrap Fusion Lumos, LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: David Hollenstein

LAB HEAD: Reiter Wolfgang

PROVIDER: PXD019646 | Pride | 2021-11-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	100531V_WR_cdc55_nostr_LysC_Kheavy_CIDETD.raw	Raw
	100531V_WR_cdc55_nostr_LysC_Kheavy_DDDT.raw	Raw
	100611V_WR_cdc55_nostr_LysC_DDDT_100505.raw	Raw
	100611V_WR_cdc55_nostr_LysC_DDDT_nocharge_100505.raw	Raw
	100618V_cdc55_nostress_LysC_K_100523_2chCID.raw	Raw

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Publications

A phosphatase-centric mechanism drives stress signaling response.

Hollenstein David Maria DM Gérecová Gabriela G Romanov Natalie N Ferrari Jessica J Veis Jiri J Janschitz Marion M Beyer Reinhard R Schüller Christoph C Ogris Egon E Hartl Markus M Ammerer Gustav G Reiter Wolfgang W

EMBO reports 20210924 11

Changing environmental cues lead to the adjustment of cellular physiology by phosphorylation signaling networks that typically center around kinases as active effectors and phosphatases as antagonistic elements. Here, we report a signaling mechanism that reverses this principle. Using the hyperosmotic stress response in Saccharomyces cerevisiae as a model system, we find that a phosphatase-driven mechanism causes induction of phosphorylation. The key activating step that triggers this phospho-pr ...[more]

PMID: 34558777

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