Proteomics

Dataset Information

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Sulfide Regulates Autophagy by Reversible Persulfidation of the Cysteine Protease ATG4


ABSTRACT: Hydrogen sulfide is a signaling molecule that regulates essential processes for plant performance, such as autophagy. In Arabidopsis thaliana, hydrogen sulfide negatively regulates autophagy independently of reactive oxygen species, but the underlying mechanism remains to be understood. To determine whether persulfidation, an emergent posttranslational modification, has a main role in the control of autophagy by sulfide, we have used a proteomic approach targeting ATG4, a cysteine protease that plays a crucial role in autophagy progression. We showed that AtATG4a from A. thaliana, which is the predominant ATG4 protease in this plant species, contains a specific site for persulfidation, the residue Cys170, included in the characteristic catalytic triad Cys-His-Asp of cysteine proteases. We tested whether persulfidation regulates the activity of ATG4 by setting up a heterologous assay using the Chlamydomonas reinhardtii CrATG8 protein as a substrate. Our findings demonstrate that sulfide significantly inactivates AtATG4a cleavage activity in a reversible manner. The biological significance of the reversible inhibition of the ATG4 protease by sulfide is supported by our findings in Arabidopsis leaves under both basal and autophagy-inducing conditions. We also observed a significant increase in the overall ATG4 activity in Arabidopsis under nitrogen starvation and osmotic stress, which is inhibited by sulfide. Therefore, our data strongly suggest that the negative regulation of autophagy by sulfide is mediated, at least, by the specific persulfidation of the protease ATG4.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Luis C. Romero  

LAB HEAD: Luis C Romero

PROVIDER: PXD019802 | Pride | 2020-12-14

REPOSITORIES: Pride

Dataset's files

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Action DRS
ALLPEAKSwtvs3ABA.xlsx Xlsx
ALLPEAKSwtvs6ABA.xlsx Xlsx
Segundalibreria12062017_0hABA1.group Other
Segundalibreria12062017_0hABA1.wiff Wiff
Segundalibreria12062017_0hABA1.wiff.scan Wiff
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Publications

Abscisic Acid-Triggered Persulfidation of the Cys Protease ATG4 Mediates Regulation of Autophagy by Sulfide.

Laureano-Marín Ana M AM   Aroca Ángeles Á   Pérez-Pérez M Esther ME   Yruela Inmaculada I   Jurado-Flores Ana A   Moreno Inmaculada I   Crespo José L JL   Romero Luis C LC   Gotor Cecilia C  

The Plant cell 20201009 12


Hydrogen sulfide is a signaling molecule that regulates essential processes in plants, such as autophagy. In Arabidopsis (<i>Arabidopsis thaliana</i>), hydrogen sulfide negatively regulates autophagy independently of reactive oxygen species via an unknown mechanism. Comparative and quantitative proteomic analysis was used to detect abscisic acid-triggered persulfidation that reveals a main role in the control of autophagy mediated by the autophagy-related (ATG) Cys protease AtATG4a. This proteas  ...[more]

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