Dataset Information

Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin

ABSTRACT: Human skin provides both physical integrity and immunological protection from the external environment, using functionally distinct layers, cell types and extracellular matrix. Despite its central role in human health and disease, the constituent proteins of skin have not been systematically characterized. Here, we combined advanced tissue dissection methods, flow cytometry and state-of-the-art proteomics to describe a spatially-resolved quantitative proteomic atlas of human skin. We quantified 10,701 proteins as a function of their spatial location and cellular origin. The resulting protein atlas and our initial data analyses demonstrate the value of proteomics for understanding cell-type diversity within the skin. We describe here the quantitative distribution of structural proteins, known and novel proteins specific to cellular subsets and those with specialized immunological funtions such as cytokines and chemokines. We anticipate this proteomic atlas of human skin will become an essential community resource for basic and translational research (www.skin.science).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Melanocyte, Dendritic Cell, Epithelial Cell, Keratinocyte, Skin, Fibroblast, Macrophage

SUBMITTER: Fabian Coscia

LAB HEAD: Matthias Mann

PROVIDER: PXD019909 | Pride | 2020-11-18

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	200619_DDA_DIA_files_annotations.xlsx	Xlsx
	20180307_QE8_nLC1_FaCo_PBMC_01.raw	Raw
	20180307_QE8_nLC1_FaCo_PBMC_02.raw	Raw
	20180307_QE8_nLC1_FaCo_PBMC_03.raw	Raw
	20180307_QE8_nLC1_FaCo_PBMC_04.raw	Raw

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Publications

Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin.

Dyring-Andersen Beatrice B Løvendorf Marianne Bengtson MB Coscia Fabian F Santos Alberto A Møller Line Bruun Pilgaard LBP Colaço Ana R AR Niu Lili L Bzorek Michael M Doll Sophia S Andersen Jørgen Lock JL Clark Rachael A RA Skov Lone L Teunissen Marcel B M MBM Mann Matthias M

Nature communications 20201105 1

Human skin provides both physical integrity and immunological protection from the external environment using functionally distinct layers, cell types and extracellular matrix. Despite its central role in human health and disease, the constituent proteins of skin have not been systematically characterized. Here, we combine advanced tissue dissection methods, flow cytometry and state-of-the-art proteomics to describe a spatially-resolved quantitative proteomic atlas of human skin. We quantify 10,7 ...[more]

PMID: 33154365

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