Dataset Information

The proteome of hand eczema assessed by tape stripping

ABSTRACT: Hand eczema (HE) is a prevalent skin disease. However, classification of HE into different subtypes remains challenging. Limited number of prior studies have employed invasive biopsy-based strategies; yet, studies of the HE proteome using non-invasive tape stripping methodology have not been reported. In this study, we wanted to assess whether global proteomic analysis of skin tape strip samples can be used for sub-classification of HE patients. Tape strips were collected from patients with HE and healthy skin. Liquid chromatography–mass spectrometry (LC/MS) proteomics was performed, and the global protein expression was analyzed. We identified 2,919 proteins in stratum corneum-derived skin cells from tape strip samples. Compared to healthy skin, the lesional samples from HE patients exhibited increased expression of immune-related markers and a decreased expression of structural barrier proteins. The difference between HE subtypes was restricted to the lesional skin areas, and included an increased expression of skin barrier-related proteins independently of the concurrent AD. In conclusion we found, that the non-invasive tape strip method used in combination with LC/MS proteomics can be used for analysis of skin protein expression in HE patients. Thus, the method shows potential for assessing the proteomic differences between subtypes of HE, and biomarker discovery.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skin

DISEASE(S): Eczema

SUBMITTER: Julie Breinholt Kjær Sølberg

LAB HEAD: Beatrice Dyring-Andersen

PROVIDER: PXD039665 | Pride | 2023-04-26

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	20201228_EXPL1_nLC14_KS_DIATapeStrips_10.raw	Raw
	20201228_EXPL1_nLC14_KS_DIATapeStrips_11.raw	Raw
	20201228_EXPL1_nLC14_KS_DIATapeStrips_12.raw	Raw
	20201228_EXPL1_nLC14_KS_DIATapeStrips_13.raw	Raw
	20201228_EXPL1_nLC14_KS_DIATapeStrips_14.raw	Raw

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The Proteome of Hand Eczema Assessed by Tape Stripping.

Sølberg Julie B K JBK Quaade Anna S AS Drici Lylia L Sulek Karolina K Ulrich Nina H NH Løvendorf Marianne B MB Thyssen Jacob P JP Mann Matthias M Dyring-Andersen Beatrice B Johansen Jeanne D JD

The Journal of investigative dermatology 20230210 8

Hand eczema (HE) is a prevalent skin disease. However, the classification of HE into different subtypes remains challenging. A limited number of previous studies have employed invasive biopsy-based strategies; yet, studies of the HE proteome using noninvasive tape-stripping methodology have not been reported. In this study, we wanted to assess whether global proteomic analysis of skin tape strip samples can be used for subclassification of patients with HE. Tape strips were collected from patien ...[more]

PMID: 36773646

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