Proteomics

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A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress

ABSTRACT: We have identified a potential selective autophagy receptor protein in Arabidopsis thaliana, C53/AT5G06830/ERP1, that is recruited to ER upon ER-stress activation and induces autophagosome formation. By affinity proteomics IP/MS with ATG8A and E, we identified this new ER-phagy receptor, which is also conserved in metazoans. With biophysical characterization, we revealed its unprecedent binding mode to ATG8 (sAIM). C53 senses proteotoxic stress in the ER lumen by forming a tripartite receptor complex with the ER-associated ufmylation ligase UFL1 and its membrane adaptor DDRGK1. However, its cargo targeted for degradation is still elusive. Initial quantitative proteomics analyses (TMT) of wild type and Atc53 mutant lines revealed that Atc53 mediates degradation of ER resident proteins as well as proteins passaging the ER to the cell wall, apoplast, and lipid droplets.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root, Leaf

SUBMITTER: Richard Imre  

LAB HEAD: Yasin Dagdas

PROVIDER: PXD019988 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20190531_1011_TAIR_Step2_noATG8_4revision_noNorm.pdResult Other
20190531_QExHFX3_RSLC2_Dagdas_Dagdas_GMI_onbead_LysC_try_dig_25per_sample_21_YFP-1.raw Raw
20190531_QExHFX3_RSLC2_Dagdas_Dagdas_GMI_onbead_LysC_try_dig_25per_sample_22_YFP-2.raw Raw
20190531_QExHFX3_RSLC2_Dagdas_Dagdas_GMI_onbead_LysC_try_dig_25per_sample_23_YFP-Tm_treated.raw Raw
20190604_QExHFX3_RSLC2_Dagdas_Dagdas_GMI_onbead_LysC_try_dig_25per_sample_27_C53_1.raw Raw
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Publications

Eukaryotes have evolved various quality control mechanisms to promote proteostasis in the endoplasmic reticulum (ER). Selective removal of certain ER domains via autophagy (termed as ER-phagy) has emerged as a major quality control mechanism. However, the degree to which ER-phagy is employed by other branches of ER-quality control remains largely elusive. Here, we identify a cytosolic protein, C53, that is specifically recruited to autophagosomes during ER-stress, in both plant and mammalian cel  ...[more]

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