Dataset Information

The effect of skyrin in normoxia and hypoxia on HCT 116 cell line

ABSTRACT: Skyrin is a plant secondary metabolite from Hypericum genus with potential in anticancer therapy. It is structurally very similar to hypericin. The mechanism of its action in normoxia as well as in hypoxia, when cells are expressing multiple adaptive mechanisms connected to poor prognosis, could be very interesting. To achieve it, label-free comparative proteomic analysis of HCT 116 cell lines samples was performed.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: David Potesil

LAB HEAD: Zbynek Zdrahal

PROVIDER: PXD019995 | Pride | 2021-04-08

REPOSITORIES: Pride

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			Action	DRS
	3503_QEx1_Babincak_120IS_top20_IT50_H00-1_2.raw	Raw
	3503_QEx1_Babincak_120IS_top20_IT50_H00-2_1.raw	Raw
	3503_QEx1_Babincak_120IS_top20_IT50_H00-3_1.raw	Raw
	3503_QEx1_Babincak_120IS_top20_IT50_H10-1_1.raw	Raw
	3503_QEx1_Babincak_120IS_top20_IT50_H10-2_2.raw	Raw

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Publications

Death Receptor 5 (TNFRSF10B) Is Upregulated and TRAIL Resistance Is Reversed in Hypoxia and Normoxia in Colorectal Cancer Cell Lines after Treatment with Skyrin, the Active Metabolite of <i>Hypericum</i> spp.

Babinčák Marián M Jendželovský Rastislav R Košuth Ján J Majerník Martin M Vargová Jana J Mikulášek Kamil K Zdráhal Zbyněk Z Fedoročko Peter P

Cancers 20210401 7

Skyrin (SKR) is a plant bisanthraquinone secondary metabolite from the <i>Hypericum</i> genus with potential use in anticancer therapy. However, its effect and mechanism of action are still unknown. The negative effect of SKR on HCT 116 and HT-29 cancer cell lines in hypoxic and normoxic conditions was observed. HCT 116 cells were more responsive to SKR treatment as demonstrated by decreased metabolic activity, cellularity and accumulation of cells in the G1 phase. Moreover, an increasing number ...[more]

PMID: 33916015

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