Proteomics

Dataset Information

0

Identification of GltB-interacting proteins by CoIP-MS

ABSTRACT: To investigate whether GltB possess more function, Co-immunoprecipitation coupled with Mass Spectrometry (CoIP-MS) was used to isolate the putative GltB binding proteins.We used strains PAO1/p-gtrS-YFP and ΔgltB/p-gtrS-YFP to ensure the membrane protein GtrS of great interest is at high levels. A target GltB-specific antibody anti-GltB was used and ΔgltB/p-gtrS-YFP was set up as a negative control. The whole gel lane was divided into small fractions and in-gel digested for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Control sample was also analyzed in parallel to distinguish the background proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Pseudomonas Aeruginosa Pao1

TISSUE(S): Cell Culture

SUBMITTER: Chenchen Xu  

LAB HEAD: Lefu Lan

PROVIDER: PXD020213 | Pride | 2021-09-09

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20180528_QE_GJ_WT_DuiZhao.raw Raw
20180528_QE_GJ_WT_ShiYan.raw Raw
txt.zip Other
Items per page:
1 - 3 of 3
altmetric image

Publications

Glucose-Binding of Periplasmic Protein GltB Activates GtrS-GltR Two-Component System in <i>Pseudomonas aeruginosa</i>.

Xu Chenchen C   Cao Qiao Q   Lan Lefu L  

Microorganisms 20210221 2

A two-component system GtrS-GltR is required for glucose transport activity in <i>P. aeruginosa</i> and plays a key role during <i>P. aeruginosa</i>-host interactions. However, the mechanism of action of GtrS-GltR has not been definitively established. Here, we show that <i>gltB</i>, which encodes a periplasmic glucose binding protein, is essential for the glucose-induced activation of GtrS-GltR in <i>P. aeruginosa</i>. We determined that GltB is capable of binding to membrane regulatory protein  ...[more]

Similar Datasets

Transcriptomics Chromatin immunoprecipitation of yeast and CoIP-chip of nucleosome-enriched chromatin after immunoprecipitation for specific factors
2009-09-25 | E-MEXP-1847 | biostudies-arrayexpress
Proteomics CMK LC-MS/MS after CoIP of ARID3A
2023-03-11 | PXD025027 | Pride
Proteomics Grass carp SERPINA1 CoIP with hepatopancreas mass spectrometry analysis
2022-08-31 | PXD036356 | Pride
Proteomics DCAF13-COIP-20231218YY0102-DDB1-E2F2
2024-09-25 | PXD056216 |
Proteomics CoIP-MS based method to identify FNIP1 binding proteins in exercising muscle
2023-11-17 | PXD046977 | Pride
Genomics RIPSeq analysis (CoIP) of CcaF1 from R. sphaeroides 2.4.1 under phototrophic growth conditions
| PRJNA957234 | ENA
Transcriptomics Single-cell RNA-seq of embryonic day 17 (E17) mouse skin epithelial cells with or without Mettl3 knockout
2020-09-04 | GSE147415 | GEO
Genomics Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
| PRJNA169138 | ENA
Proteomics A0913 interacts with CP47 of PSII and ApcF of PBS
2024-11-06 | PXD057430 | Pride
Other Identification of RNA molecules associated with the RNA-chaperone Hfq
2008-08-23 | GSE10149 | GEO