Proteomics

Dataset Information

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Correction of indels in the atpB gene by genetic recoding in the chloroplast of Oenothera and tobacco

ABSTRACT: Genetic recoding, a process that modifies genome templated protein sequence, plays an important role especially in the small genomes of viruses and bacteria. Chloroplasts have evolved from cyanobacterial ancestry and mainly retain the prokaryotic gene expression machinery. I-iota, a plastome mutant of Oenothera (the evening primrose), carries a single adenine insertion in a polyA stretch near to the 5’-end of the atpB gene, encoding the β-CF1 subunit of the ATP synthase This causes formation of a truncated protein. Surprisingly, a full-length AtpB protein is detectable in I-iota leaves, suggesting the presence of genetic recoding operating in I-iota. In order to analyze this phenomenon in more detail, several tobacco transplastomic lines were generated inducing and destroying genetic recoding of atpB. We identified that a two adenine (+2A) insertion was more efficiently compensated in plants than +1A, -1A or -2A mutations at the same location. Furthermore, a homopolymeric composition of the polyA stretch is essential for successful recoding: plants carrying a destroyed polyA stretch have an albino-phenotype, indicating the absence of genetic recoding. Here we show evidence for genetic recoding acting in the chloroplast that plays presumablya compensatory function and enables correction of indels in an essential gene for autotrophic growth.

INSTRUMENT(S): TSQ Vantage

ORGANISM(S): Oenothera Elata

TISSUE(S): Leaf

SUBMITTER: Witold Szymanski  

LAB HEAD: Dr. Search Results Web results Stephan Greiner

PROVIDER: PXD020246 | Pride | 2022-02-15

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
atp_bsa_3.vws Other
atp_bsanew.xlsx Xlsx
iota_sp_1-1.RAW Raw
iota_sp_2-1.RAW Raw
iota_sp_3-1.RAW Raw
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Publications

Correction of frameshift mutations in the atpB gene by translational recoding in chloroplasts of Oenothera and tobacco.

Malinova Irina I   Zupok Arkadiusz A   Massouh Amid A   Schöttler Mark Aurel MA   Meyer Etienne H EH   Yaneva-Roder Liliya L   Szymanski Witold W   Rößner Margit M   Ruf Stephanie S   Bock Ralph R   Greiner Stephan S  

The Plant cell 20210701 5

Translational recoding, also known as ribosomal frameshifting, is a process that causes ribosome slippage along the messenger RNA, thereby changing the amino acid sequence of the synthesized protein. Whether the chloroplast employs recoding is unknown. I-iota, a plastome mutant of Oenothera (evening primrose), carries a single adenine insertion in an oligoA stretch [11A] of the atpB coding region (encoding the β-subunit of the ATP synthase). The mutation is expected to cause synthesis of a trunc  ...[more]