Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

C-Man peptide pulldowns from Brain

ABSTRACT: Peptide pulldowns with C-man antibodies to demostrate the utelity of reagents for proteomic analysis. Using Murine brain samples Novel C-man sites are readily identifiable.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Nichollas Scott

LAB HEAD: Ethan Goddard-Borger

PROVIDER: PXD020336 | Pride | 2020-11-16

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	200526_Nsco_EBS_Cman_pulldown_CMT_B1_20200528162416.raw	Raw
	200526_Nsco_EBS_Cman_pulldown_CMT_B2.raw	Raw
	200526_Nsco_EBS_Cman_pulldown_CMT_B2_20200528234434.raw	Raw
	200526_Nsco_EBS_Cman_pulldown_CMT_B3.raw	Raw
	200526_Nsco_EBS_Cman_pulldown_CMT_B3_20200529070450.raw	Raw

Items per page:

1 - 5 of 27

Similar Datasets

Nucleocytoplasmic O-Man glycoproteome

Project description:Dynamic cycling of N-Acetylglucosamine (GlcNAc) on serine (Ser) and threonine (Thr) residues (O-GlcNAcylation) is an essential process in all eukaryotic cells except yeast, e.g Saccharomyces cerevisiae. O-GlcNAcylation modulates signaling and cellular processes in an intricate interplay with protein phosphorylation, and serves as a key sensor of nutrients by linking the hexosamine biosynthetic pathway (HBP) to cellular signaling. A longstanding conundrum has been how yeast survives without O-GlcNAcylation in light of its similar phosphorylation signaling system. We previously developed a sensitive lectin enrichment and mass spectrometry workflow for identification of the human O-Mannose (O-Man) glycoproteome, and used this to identify a pleothora of O-linked mannose glycoproteins in human cell lines including the large family of cadherins and protocadherins. Here, we applied the workflow to yeast with the aim to characterize the yeast O-Man glycoproteome, and doing so we discovered hitherto unknown O-Man glycosites on nuclear, cytoplasmic and mitochondrial proteins in Saccharomyces cerevisiae. The type of nucleocytoplasmic proteins and the localization of identified O-Man residues mirrors that of the O-GlcNAc glycoproteome in other eukaryotic cells, indicating that the two different types of O-glycosylations serve the same important biological functions. The discovery opens for exploration of the enzyme machinery that is predicted to regulate the discovered nucleocytoplasmic O-Man glycosylation. It is likely that manipulation of this type of O-Man glycosylation will have wide applications for yeast bioprocessing.

2015-12-08 | PXD002924 | Pride

Repo-Man/PP1 regulates heterochromatin formation in interphase

Project description:Repo-Man chromatin binding sites were obtained by expression of GST:Repo-Man and incubation with nucleosomes extracted from HeLa cells (GST alone signal was used as a negative control and subtracted from the GST:Repo-Man chromatin bound fraction)

2016-11-23 | GSE84035 | GEO

Global view of domain-specific O-linked mannose glycosylation in glycoengineered cells

Project description:Protein O-linked mannose (O-Man) glycosylation is an evolutionary conserved post-translational modification, whose biosynthesis is initiated by three non-redundant enzyme families, POMT1/POMT2, TMTC1-4 and TMEM260. In this study, we applied a targeted workflow for membrane glycoproteomics to five human cell lines and to a panel of genetically engineered cells with individual and combinatorial knock-out of O-Man glycosyltransferase genes to extensively map substrates, specificities, and crosstalk of O-Man enzymes. Our quantitative glycoproteomics results demonstrate new protein targets for the POMT1/POMT2 pathway and show that TMTC1-4 and TMEM260 widely target distinct Ig-like protein domains of plasma membrane proteins. This new evidence adds further knowledge on the emerging concept of domain-specific O-Man glycosylation and establishes a platform for functional studies of O-Man glycosylated adhesion molecules and receptors.

2024-06-10 | PXD045597 | Pride

ANCA Glomerulonephritis: From Molecules to Man

Project description:ANCA Glomerulonephritis: From Molecules to Man

| PRJNA671246 | ENA

Repo-Man/PP1 regulates heterochromatin formation in interphase

Project description:GST:Repo-Man(C-terminus) or GST:alone were expressed as in Vagnarelli et al. 2011. Before elution, beads were first incubated with nucleosomes extracted from HeLa. HeLa cells were lysed and nucleosomes were digested with MNase (20min, 37C). Nucleosomes were firstly incubated with GST alone for 2hrs. This cleared lysate was then incubated with GST:Repo-Man and GST alone. Beads were then eluted and mix with 3x Laemli Buffer. Histone bands were sent to Mass Spec.

2016-12-07 | PXD004613 | Pride

Secreted footprint of pluripotency in Man-13 HeSCs

Project description:With a view to developing a much-needed non-invasive method for monitoring the healthy pluripotent state of human stem cells in culture, we undertook proteomic analysis of the waste medium from cultured induced (Rebl.PAT) human pluripotent stem cells (hPSCs). Cells were grown in E8 medium to maintain pluripotency, and then transferred to FGF2 and TGFβ deficient E6 media for 48 hours to replicate an early, undirected dissolution of pluripotency. We identified a distinct proteomic footprint associated with early loss of pluripotency in both hPSC lines, and a strong correlation with changes in the transcriptome. We demonstrate that multiplexing of four E8- against four E6- enriched secretome biomarkers provides a robust, diagnostic metric for pluripotent state. These biomarkers were further confirmed by Western blotting which demonstrated consistent correlation with the pluripotent state across cell lines, and in response to a recovery assay.

2024-03-26 | PXD045525 | Pride

Corroborating written history with ancient DNA: the case of the Well-man described in an Old Norse saga

Project description:Sverresborg Well Man genomic sequencing

| PRJEB69227 | ENA

GNPS - 3D molecular analysis of skin surface_Time_Course_Volunteer 5-man-Face. MS/MS Data

Project description:mzXML files of MS/MS data from skin swab samples of 1 man volunteer. Samples were collected from 17 spots on a man face, everyday during 10 days. MS/MS data collected from beauty products used by this subject are also included.

2014-05-27 | MSV000078683 | GNPS

Inventory of genes expressed in human control hippocampus

Project description:Serial analysis of gene expression (SAGE) was used to get a global view of the gene profile in human hippocampus. A library were generated from control hippocampus, obtained by rapid autopsy. Keywords: hippocampus human inventory genes Control hippocampus (used to construct the SAGE control library) was obtained from a 48 years old man without history of seizures or other neurological diseases and no brain abnormalities at autopsy and histologically normal hippocampus. Autopsy was performed within 4 hours after death. Tissue was snap-frozen and stored at –80 0C until use. Total RNA was isolated from control hippocampus and hippocampal surgical specimens, using the Trizol method according to the manufacturer’s instructions (Invitrogen - Life Technologies, The Netherlands). Part of the anterior hippocampus of control (including sectors CA1- CA4 and dentate gyrus, DG) was used. Poly(A)+ RNA isolation, cDNA synthesis and all subsequent steps of the SAGE procedure were essentially performed as described (Velculescu et al., 1995) with minor modifications given previously (Michiels et al., 1999).

2005-12-29 | E-GEOD-3928 | biostudies-arrayexpress

Single-cell transcriptomics reveals natural product Shi-Bi-Man promotes hair regeneration

Project description:Single-cell transcriptomics reveals natural product Shi-Bi-Man promotes hair regeneration

| PRJNA806791 | ENA