Dataset Information

Mapping sites of O-fucose modification on mouse NOTCH2

ABSTRACT: The O-fucose modification sites on mouse NOTCH2 were determined by mass spectral glycoproteomic site mapping. The Extracellular Domain of mouse NOTCH2 was produced in HEK293T cells in the presence or absence of the three Fringe enzymes: Lunatic fringe, Manic fringe, or Radical fringe. The protein was purified from the medium, reduced and alkylated, digested with trypsin, chymotrypsin or V8 protease, and the resulting peptides were analyzed by nano-LC mass spectrometry. The majority of the sites were identified using an Agilent model 6340 Ion Trap mass spectrometer. A few sites were identified using a Q-Exactive Plus mass spectrometer.

INSTRUMENT(S): 6340 Ion Trap LC/MS, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Robert Haltiwanger

LAB HEAD: Robert S. Haltiwanger

PROVIDER: PXD020431 | Pride | 2020-09-11

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	020216_EGF1_36_LFNG_Trypsin.yep	Other
	020216_EGF1_36_MFNG_Trypsin.yep	Other
	020216_EGF1_36_NOFNG_Trypsin.yep	Other
	020216_EGF1_36_RFNG_Trypsin.yep	Other
	021116_EGF1_36_LFNG_Chymotrypsin.yep	Other

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Publications

Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2.

Kakuda Shinako S LoPilato Rachel K RK Ito Atsuko A Haltiwanger Robert S RS

The Journal of biological chemistry 20200819 43

Notch signaling is a cellular pathway regulating cell-fate determination and adult tissue homeostasis. Little is known about how canonical Notch ligands or Fringe enzymes differentially affect NOTCH1 and NOTCH2. Using cell-based Notch signaling and ligand-binding assays, we evaluated differences in NOTCH1 and NOTCH2 responses to Delta-like (DLL) and Jagged (JAG) family members and the extent to which Fringe enzymes modulate their activity. In the absence of Fringes, DLL4-NOTCH1 activation was mo ...[more]

PMID: 32820046

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