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Proteome analysis reveals a significant host-specific response in Rhizobium leguminosarum bv viciae endosymbiotic cells

ABSTRACT: We study the proteomic profile of endosymbiotic cells (bacteroids) induced by R. leguminosarum bv viciae strain UPM791 in nodules from two different hosts. Comparative analysis of bacteroids induced in pea and lentil nodules revealed the existence of a significant host-specific differential response affecting multiple bacterial proteins, and also revealed the presence of different sets of plant-derived nodule-specific cysteine rich (NCR) peptides.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rhizobium Leguminosarum Bv. Viciae

TISSUE(S): Root

SUBMITTER: Jose Palacios

LAB HEAD: Jose Palacios

PROVIDER: PXD020631 | Pride | 2020-11-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	040616_Lentil3.raw	Raw
	060516_Lentil2.mgf	Mgf
	060516_Lentil2.raw	Raw
	060516_Lentil2_Fabaceae.msf	Msf
	060516_Lentil2_NCRs_Lentil.msf	Msf

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Publications

Proteome Analysis Reveals a Significant Host-Specific Response in Rhizobium leguminosarum bv. viciae Endosymbiotic Cells.

Durán David D Albareda Marta M García Carlos C Marina Ana-Isabel AI Ruiz-Argüeso Tomás T Palacios Jose-Manuel JM

Molecular & cellular proteomics : MCP 20201206

The Rhizobium-legume symbiosis is a beneficial interaction in which the bacterium converts atmospheric nitrogen into ammonia and delivers it to the plant in exchange for carbon compounds. This symbiosis implies the adaptation of bacteria to live inside host plant cells. In this work, we apply RP-LC-MS/MS and isobaric tags as relative and absolute quantitation techniques to study the proteomic profile of endosymbiotic cells (bacteroids) induced by Rhizobium leguminosarum bv viciae strain UPM791 i ...[more]

PMID: 33214187

Similar Datasets

Host-dependent NCR profiles of Rhizobium leguminosarum bacteroids induced in pea and lentil plants.

Project description:We have determined the profile of plant-derived NCR peptides present in endosymbiotic cells (bacteroids) induced by R. leguminosarum bv viciae strain UPM791 in nodules from two different hosts (pea and lentil).

2020-11-27 | PXD020634 | Pride

Transcription profiling of bacteroid Rhizobium leguminosarum strain 3841 wild type vs bacA mutant harvested from pea plant nodules

Project description:Wildtype bacteroids of Rhizobium leguminosarum biovar viciae 3841 were compared to a bacA mutant. In both cases bacteroids were harvested from 7 day old nodules from pea plants.

2009-01-06 | E-MEXP-1966 | biostudies-arrayexpress

Proteomic screening for potentially amyloidogenic proteins of Rhizobium leguminosarum in effective and ineffective root nodules of Pisum sativum L.

Project description:We studied potentially amyloidogenic proteins (e.g. protein forming polymers and complexes that are resistant to treatment with ionic detergents) in root nodules formed by two lines of garden pea (P. sativum L.): Sprint-2 (Fix+ phenotype) and Sprint-2Fix- (sym31) (Fix- phenotype) inoculated with the Rhizobium leguminosarum bv. viciae RCAM1026 root nodule bacteria. The Fix+ phenotype is characterized by effective (ability to fix nitrogen) root nodules formation. The Fix- line is a descendant of the Fix+ line and forms ineffective root nodules (unable to fix nitrogen) with undifferentiated bacteroids. We demonstrated the presence of both plant and bacterial proteins in detergent resistant fractions, including previously identified amyloid proteins RopA and RopB of R. leguminosarum and vicilin of P. sativum L.

2022-10-25 | PXD035724 | Pride

Transcription profiling of Rhizobium leguminosarum biovar viciae strain 3841 inoculated onto vetch seeds compared to cells grown in free-living culture on succinate ammonia AMS

Project description:Rhizobium leguminosarum biovar viciae strain 3841 was inoculated onto Viccia cracca seeds and bacteroids were harvested at 28d. Gene expression was then compared to free-living Rhizobium leguminosarum biovar viciae strain 3841 grown on succinate ammonia AMS.

2008-11-30 | E-MEXP-1924 | biostudies-arrayexpress

Antioxidant mechanism of glutaredoxins and their role in symbiotic nitrogen fixation in Rhizobium leguminosarum bv. viciae 3841

Project description:Glutaredoxins (Grx) are redoxin family proteins that reduce disulfifides and mixed disulfifides between glutathione and proteins. Rhizobium leguminosarum 3841 contains three genes coding for glutaredoxins: RL4289 (grxA) codes for a dithiolic glutaredoxin, RL2615 (grxB) codes for a monothiol glutaredoxin, while RL4261 (grxC) codes for a glutaredoxin-like NrdH protein. In this study, we have generated mutants which have interrupted one, two, or three glutaredoxin genes. These mutants had no different growth phenotypes from the wild type RL3841. Mutation of grxC did not affect R. leguminosarum antioxidant or symbiotic capacities, while grxA-derived or grxB-derived mutants decreased the antioxidant and fixation nitrogen capacity. grxA-derived mutants was severely impaired in there rhizosphere colonization, and formed maller nodules with defects of bacteroid differentiation. whereas nodules induced by grxB-derived mutants contained abnormally thick cortexs and prematurely senescent bacteroids. Surprisingly the grx triple mutant presented an enhanced defect in antioxidant and symbiotic capacities of R. leguminosarum. LC-MS/MS analysis quantitative proteomics techniques were employed to compare differential grx triple mutant root bacteroids in response to the wild type infection. 137 differentially expressed proteins were identified including 56 up-regulated and 81 down-regulated proteins. By sorting the identified proteins according to metabolic function, twenty-eight proteins were involved in transporter activity, twenty proteins were related to stress response and virulence, and sixteen proteins were related to amino acid metabolism. Overall, R. leguminosarum glutaredoxins play an important role in root nodule symbiosis by involving the functions of anti-oxidant defenses, Fe-S cluster assembly, and control of bacteroid differentiation and senescence.

2020-12-02 | PXD017835 | Pride

A GMC Oxidoreductase GmcA is required for symbiotic nitrogen fixation in Rhizobium leguminosarum bv. Viciae

Project description:GmcA is a FAD-containing enzyme belonging to the GMC (glucose-methanol-choline oxidase) family of oxidoreductases. A mutation in the R. leguminosarum gmcA gene was generated by homologous recombination. The mutation in gmcA did not affect the growth of R. leguminosarum, but it displayed decreased antioxidative capacity at H2O2 conditions higher than 5 mM. The gmcA mutant strain displayed no difference of glutathione reductase activity, but significantly lower level of the glutathione peroxidase activity than the wild type. Although the gmcA mutant can induce the formation of nodules, the symbiotic ability was severely impaired, which led to an abnormal nodulation phenotype coupled to a 30% reduction in the nitrogen fixation capacity. The observation on ultrastructure of 4-week pea nodules showed that the mutant bacteroids tended to start senescence earlier, and accumulate poly-β-hydroxybutyrate (PHB) granules. In addition, the gmcA mutant was severely impaired in rhizosphere colonization. Real-time quantitative PCR showed that the gmcA gene expression is significantly upregulated in all the detected stages of nodule development, and statistically significant decreases in the expression of the redoxin genes katG, katE and ohrB, were found in gmcA mutant bacteroids. LC-MS/MS analysis quantitative proteomics techniques were employed to compare differential gmcA mutant root bacteroids in response to the wild type infection. Sixty differentially expressed proteins were identified including 33 up-regulated and 27 down-regulated proteins. By sorting the identified proteins according to metabolic function, fifteen proteins were transporter protein, twelve proteins were related to stress response and virulence, and nine proteins were related to transcription factor activity. Moreover, nine proteins related to amino acid metabolism were over-expressed.

2020-02-24 | PXD017485 | Pride

Rhizobium leguminosarum bv. viciae WSM1455

Project description:Rhizobium leguminosarum bv. viciae WSM1455 genome sequencing

| PRJNA63183 | ENA

Transcription profiling of Rhizobium leguminosarum biovar viciae strain 3841 inoculated onto pea seeds compared to cells grown in free-living culture on succinate ammonia AMS

Project description:Rhizobium leguminosarum bv viciae strain 3841 was inoculated onto pea (Pisum sativum) seeds and nodules were harvested at 28 d. The gene expression was compared to free-living bacteria grown on succinate ammonia AMS medium.

2008-11-30 | E-MEXP-1925 | biostudies-arrayexpress

Rhizobium leguminosarum bv. viciae strain:3841

Project description:Rhizobium leguminosarum bv. viciae strain:3841 Epigenomics

| PRJNA243966 | ENA

Succinate transport is not essential for symbiotic nitrogen fixation by Sinorhizobium meliloti nor Rhizobium leguminosarum

Project description:Symbiotic nitrogen fixation (SNF) is an energetically expensive process performed by bacteria known as rhizobia during endosymbiotic relationships with leguminous plants. The bacteria require the plant to provide a carbon source for generation of the reductant to power SNF. Although it is well known that C4-dicarboxylates (succinate, fumarate, malate) function as the primary, if not sole, carbon source provided to the rhizobia, the relative contribution of each C4-dicarboxylate is not known. Here, we employ genetic and systems-level analyses to address this issue. Expression of a malate specific transporter (MaeP) in Sinorhizobium meliloti Rm1021 dct mutants unable to transport C4-dicarboxylates resulted in malate import rates up to ~ 30% that of wild type S. meliloti. This was sufficient to support SNF with Medicago sativa, with acetylene reduction rates up to ~ 50% those of plants inoculated with wild type S. meliloti. Rhizobium leguminosarum bv. viciae 3841 dct mutants unable to transport C4-dicarboxylates but expressing the maeP transporter had strong symbiotic properties, with Pisum sativum plants inoculated with these strains appearing similar to plants inoculated with wild type R. leguminosarum. This was despite malate transport rates by the mutant bacteroids being < 10% those of the wild type. A transcriptomics analysis of the combined plant/bacterium nodule transcriptome was performed using RNA-sequencing to identify any systems-level adaptations in response to the inability of the bacteria to import succinate or fumarate. Few transcriptional changes, with no obvious pattern, were revealed by this analysis. Overall, these data illustrated that succinate and fumarate are not essential for SNF, and that at least in specific symbioses, L-malate is likely to naturally serve as the primary C4-dicarboxylate provided to the bacterium.

2017-09-16 | GSE101430 | GEO

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