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Influenza viral RNA pulldown MS in THP-1 cells

ABSTRACT: Due to the RNA nature of their genomes, influenza viruses have to utilize many RNA-binding proteins (RBPs) of both viral and host origin, for their replication. To uncover the comprehensive vRNA-host protein interactions, we performed affinity purification coupled with mass spectrometry (AP-MS) analysis of influenza vRNA complexes. The eight vRNA segments of H7N9 were transcribed and individually labeled with biotin in vitro and incubated with IAV virus-infected THP-1 cells, and vRNA complexes were enriched streptavidin magnetic beads and analyzed by mass spectrometry

INSTRUMENT(S): Q TRAP

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Zhimin Jiang

LAB HEAD: Liu jinhua

PROVIDER: PXD020723 | Pride | 2021-03-25

REPOSITORIES: Pride

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			Action	DRS
	Emptybeadsfornegativecontrol.mzid.gz	Mzid
	Emptybeadsfornegativecontrol.xml	Xml
	F002853.mgf	Mgf
	F002854.mgf	Mgf
	F002855.mgf	Mgf

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