Proteomics

Dataset Information

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Study of human gut microbiota from healthy adults

ABSTRACT: The link between the gut microbiota of a human being (a complex group of microorganism including not only bacteria but also fungi, viruses, etc.,) that form an ecosystem in his gastrointestinal tract and his physiological state is nowadays unquestionable. Metaproteomics has emerged as a useful technique to characterize this microbial community, not just taxonomically, but also focusing on specific biological processes carried out by gut microbiota that may have an effect in the host health or pathological state. In order to characterize this host-microbiota inter-relation, we carried out the metaproteomic study of 6 stool samples from 6 healthy adults. A total of 37 080 peptide sequences and 10 686 protein groups were identified in this study. Regarding taxonomic information, we found a total of 247 taxa among 105 were species. Interesting contributions of microbiota metabolism to human host physiology has also been described.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Microbiota Homo Sapiens (human)

TISSUE(S): Gastrointestinal Tract

SUBMITTER: Concha Gil  

LAB HEAD: Concha Gil

PROVIDER: PXD020786 | Pride | 2021-07-06

REPOSITORIES: Pride

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18-164_Muestra1-001.raw Raw
18-164_Muestra2-005.raw Raw
18-164_Muestra3-002.raw Raw
18-164_Muestra4-003.raw Raw
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Publications

Distinct Human Gut Microbial Taxonomic Signatures Uncovered With Different Sample Processing and Microbial Cell Disruption Methods for Metaproteomic Analysis.

García-Durán Carmen C   Martínez-López Raquel R   Zapico Inés I   Pérez Enrique E   Romeu Eduardo E   Arroyo Javier J   Hernáez María Luisa ML   Pitarch Aida A   Monteoliva Lucía L   Gil Concha C  

Frontiers in microbiology 20210705

The use of metaproteomics for studying the human gut microbiota can shed light on the taxonomic profile and the functional role of the microbial community. Nevertheless, methods for extracting proteins from stool samples continue to evolve, in the pursuit of optimal protocols for moistening and dispersing the stool sample and for disrupting microbial cells, which are two critical steps for ensuring good protein recovery. Here, we evaluated different stool sample processing (SSP) and microbial ce  ...[more]

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