Proteomics

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Evaluation of different protein extraction protocols to study human gut microbiota.

ABSTRACT: The link between the gut microbiota and the human physiological state has been demonstrated in recent years. High gut microbiota diversity has been linked to many beneficial functions necessary or human health, while dysbiosis has been correlated to different pathological states. In this context, the study of the gut microbiota results of high relevance been necessary the development of different techniques capable of characterizing this complex ecosystem. Metaproteomics has been proved useful in the characterization of complex protein samples becoming a suitable tool for the study of these microbial communities. However, due to the complexity of these samples, protein extraction protocols may affect metaproteomics results. In this context, we evaluated stool sample processing (SSP) and microbial cell disruption, assessing the impact of different protocol modifications in the number of peptides and proteins identified. We compared different stool processing conditions and microbial cell disruption methods in terms of protein and peptide identifications and taxonomic profiles.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Microbiota Homo Sapiens (human)

TISSUE(S): Gastrointestinal Tract

SUBMITTER: Concha Gil  

LAB HEAD: Concha Gil

PROVIDER: PXD025659 | Pride | 2021-07-06

REPOSITORIES: Pride

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Publications

Distinct Human Gut Microbial Taxonomic Signatures Uncovered With Different Sample Processing and Microbial Cell Disruption Methods for Metaproteomic Analysis.

García-Durán Carmen C   Martínez-López Raquel R   Zapico Inés I   Pérez Enrique E   Romeu Eduardo E   Arroyo Javier J   Hernáez María Luisa ML   Pitarch Aida A   Monteoliva Lucía L   Gil Concha C  

Frontiers in microbiology 20210705

The use of metaproteomics for studying the human gut microbiota can shed light on the taxonomic profile and the functional role of the microbial community. Nevertheless, methods for extracting proteins from stool samples continue to evolve, in the pursuit of optimal protocols for moistening and dispersing the stool sample and for disrupting microbial cells, which are two critical steps for ensuring good protein recovery. Here, we evaluated different stool sample processing (SSP) and microbial ce  ...[more]

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