Dataset Information

Investigating byssogenesis with proteomic analysis of byssus, foot and mantle in Mytilus mussels by LC-MS/MS

ABSTRACT: Mussels synthesize an interesting class of biological materials with unique properties to adhere onto virtually any solid surface. Inter- and cross-species analyses have revealed that mussel byssus fabrication is influenced by environmental factors. In this study, proteins expressed specifically byssus and in the byssal-producing organ (foot) were examined for two mussel species from the Mytilidae family using liquid chromatography coupled with high-resolution tandem mass spectrometry (LC-MS/MS). The main goal was to describe which proteins are exclusively expressed in the mussel's foot gland, which is responsible for byssus secretion. Proteins uniquely found in the foot samples have been found by comparing to a control tissue (mantle). The overlap with proteins found in byssus samples were also investigated. A restricted list of these proteins have been highlighted as byssus-related, believed to contribute to the unique properties of byssus, such as securing adhesion and toughness, and plasticity based on environmental factors (including promoter regulation). This work may provide further directions on thread biofabrication and additional information on its regulation, as well as contribute to a more complete description of the mussel proteome.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mytilus Californianus Mytilus Edulis (blue Mussel)

SUBMITTER: Lekha Sleno

LAB HEAD: Lekha Sleno

PROVIDER: PXD021023 | Pride | 2020-09-17

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	ByssusProteomics.sf3	Other
	IdentificationMYTCA_Foot_TP-co_A1.mzid.mzid.gz	Mzid
	IdentificationMYTCA_Foot_TP-co_A1.mzid.mzid_Identification_MYTCA_Foot_TP-co_A1.mzid.MGF	Mzid
	IdentificationMYTCA_Foot_TP-co_A2.mzid.mzid.gz	Mzid
	IdentificationMYTCA_Foot_TP-co_A2.mzid.mzid_Identification_MYTCA_Foot_TP-co_A2.mzid.MGF	Mzid

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Publications

Investigating Byssogenesis with Proteomic Analysis of Byssus, Foot, and Mantle in Mytilus Mussels by LC-MS/MS.

Sansoucy Maxime M Tremblay Réjean R Carrington Emily E Marcotte Isabelle I Sleno Lekha L

Proteomics 20201211 2

Mussel byssus represents a fascinating class of biological materials with a unique capacity to adhere onto virtually any solid surface. Proteins expressed in byssus, the byssal-producing organ (foot) as well as mantle tissue from Mytilus edulis or Mytilus californianus are analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The mantle is used as a control tissue to pinpoint unique proteins from the foot samples potentially involved in byssogenesis. This work represents an impo ...[more]

PMID: 32910497

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