Dataset Information

The haemolymph proteome of Mytilus edulis: insights into the circulatory system and innate immunity of marine mussels

ABSTRACT: Mussels are a group of sessile and filter-feeding invertebrates, and are key structural elements of many marine and freshwater ecosystems. The mussel haemolymph is the first defense line to pathogen infection and inflammation with an innate immune response. Despite the importance of this body fluid in determining the physiological condition of the animal, little is known about the molecular mechanisms underlying the cellular and humoral responses. Proteomic characterization of this body fluid has been hampered by the scarce Mytilus sp genomic data available. To systematic characterize the marine mussel Mytilus edulis hemolymph proteome, we have applied a mass spectrometry (nanoLC-MS/MS) strategy integrating genomics and transcriptomics data for proteins identification. After sample analysis and first identification based on MS/MS data comparison, proteins with unknown functions were annotated with blast using public database (nrNCBI) information. Overall 654 haemolymph proteins were identified with high confidence. The gene ontology analysis revealed that the majority of haemolymph proteins participate in primary cellular metabolic processes: energy production and metabolism of biomolecules. Nevertheless it also revealed a protein complement whose functions could be related to oxidative stress defense, xenobiotic detoxification, drug metabolism and immune response.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mytilus Edulis (blue Mussel)

TISSUE(S): Blood Serum, Blood

SUBMITTER: Alexandre Campos

LAB HEAD: Susana Cristobal

PROVIDER: PXD001951 | Pride | 2015-09-14

REPOSITORIES: pride

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Shotgun analysis of the marine mussel Mytilus edulis hemolymph proteome and mapping the innate immunity elements.

Campos Alexandre A Apraiz Itxaso I da Fonseca Rute R RR Cristobal Susana S

Proteomics 20151013 23-24

The marine mussel innate immunity provides protection to pathogen invasion and inflammation. In this regard, the mussel hemolymph takes a main role in the animal innate response. Despite the importance of this body fluid in determining the physiological condition of the animal, little is known about the molecular mechanisms underlying the cellular and humoral responses. In this work, we have applied a MS (nano-LC-MS/MS) strategy integrating genomic and transcriptomic data with the aim to: (i) id ...[more]

PMID: 26351202

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			Action	DRS
	37.mzid.gz	Mzid
	37.mzid.gz_37.MGF	Mzid
	37.mzid.gz_37.pride.mgf.gz	Mzid
	37.pride.mztab.gz	Mztab
	37.raw	Raw