Dataset Information

Uncovering the secretome of mesenchymal stromal cells exposed to healthy, traumatic and 1 degenerative intervertebral discs: A proteomic analysis

ABSTRACT: Mesenchymal stromal cells (MSCs) have been introduced as promising cell source for regenerative medicine. Besides their multilineage differentiation capacity, MSCs release a wide spectrum of bioactive factors. This secretome holds immunomodulatory and regenerative capacities. In intervertebral disc (IVD) cells, application of MSC secretome has been shown to decrease the apoptosis rate, induce proliferation and promote production of extracellular matrix (ECM). For clinical translation of secretome-based treatment, characterization of the secretome composition is needed to better understand the induced biological processes and identify potentially effective secretomes. Methods: This study aimed to investigate the proteome released by bone marrow derived MSCs following exposure to a healthy, traumatic, or degenerative human IVD environment by mass spectroscopy and quantitative immunoassay analyses. Exposure of MSCs to the proinflammatory stimulus interleukin 1β (IL-1b) was used as control.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Witold Wolski

LAB HEAD: Sibylle Grad

PROVIDER: PXD021281 | Pride | 2021-09-09

REPOSITORIES: Pride

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Source:

			Action	DRS
	20190118_003_S190199_137_S_14.raw	Raw
	20190118_004_S190210_199_S_12.raw	Raw
	20190118_005_S190233_58_S_11.raw	Raw
	20190118_006_S190193_132_S_11.raw	Raw
	20190118_009_S190214_210_S_12.raw	Raw

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Uncovering the secretome of mesenchymal stromal cells exposed to healthy, traumatic, and degenerative intervertebral discs: a proteomic analysis.

Wangler Sebastian S Kamali Amir A Wapp Christina C Wuertz-Kozak Karin K Häckel Sonja S Fortes Claudia C Benneker Lorin M LM Haglund Lisbet L Richards R Geoff RG Alini Mauro M Peroglio Marianna M Grad Sibylle S

Stem cell research & therapy 20210107 1

<h4>Background</h4>Mesenchymal stromal cells (MSCs) have been introduced as promising cell source for regenerative medicine. Besides their multilineage differentiation capacity, MSCs release a wide spectrum of bioactive factors. This secretome holds immunomodulatory and regenerative capacities. In intervertebral disc (IVD) cells, application of MSC secretome has been shown to decrease the apoptosis rate, induce proliferation, and promote production of extracellular matrix (ECM). For clinical tra ...[more]

PMID: 33413584

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