Dataset Information

Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation.

ABSTRACT: Protein composition of human ovarian follicular fluid (FF) constitutes the microenvironment for oocyte development. Several proteomics studies of FF from pre-ovulatory follicles have revealed insights on oocyte maturation, however, there is a lack of knowledge on changes produced at protein levels in the FF of human small antral follicles (hSAF) related to the upcoming oocyte maturation. Using mass spectrometry-based proteomics, we evaluated the protein composition of FF that surrounds oocytes capable to reach metaphase II (MII) after IVM with the protein profile of FF that surrounds immature oocytes. The samples were collected from small antral follicles (size 6.0 ± 1.5 mm) extracted from six women, from which two or three samples were extracted. The comparison was based on both, a multivariate (sPLS-DA) and univariate analyses (t-test).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Follicular Fluid

DISEASE(S): Female Reproductive System

SUBMITTER: Indira Pla Parada

LAB HEAD: Johan Malm

PROVIDER: PXD021338 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20200305_FF_Dep_small_R3.msf	Msf
	20200305_FF_Dep_small_R3.raw	Raw
	20200305_FF_Dep_small_R4.raw	Raw
	20200305_FF_Dep_small_R5.raw	Raw
	20200305_FF_M_43_DDA.raw	Raw

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Publications

Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation.

Pla Indira I Sanchez Aniel A Pors Susanne Elisabeth SE Pawlowski Krzysztof K Appelqvist Roger R Sahlin K Barbara KB Poulsen Liv La Cour LC Marko-Varga György G Andersen Claus Yding CY Malm Johan J

Human reproduction (Oxford, England) 20210201 3

<h4>Study question</h4>Is it possible to identify by mass spectrometry a wider range of proteins and key proteins involved in folliculogenesis and oocyte growth and development by studying follicular fluid (FF) from human small antral follicles (hSAF)?<h4>Summary answer</h4>The largest number of proteins currently reported in human FF was identified in this study analysing hSAF where several proteins showed a strong relationship with follicular developmental processes.<h4>What is known already</ ...[more]

PMID: 33313811

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Project description:Dibutyl phthalate (DBP), di-2-ethylhexyl phthalate (DEHP), and benzyl butyl phthalate (BBP) are three phthalates commonly found in consumer products, including the plastic coating of pharmaceuticals and personal care products. Folliculogenesis, a tightly regulated process occurring in the ovary, is the maturation of an immature primordial follicle to a mature antral follicle. Follicles house the oocyte and antral follicles specifically play a crucial role in ovarian steroidogenesis and ovulation. DBP, BBP, and DEHP have been associated with inhibited antral follicle growth in vitro, decreased ovulation rates in vitro, and decreased antral follicle counts in women. However, little is known about the effects of a three-phthalate mixture on antral follicles in vivo. The objective of this study was to evaluate the effects of a human relevant mixture of DBP, BBP, and DEHP on ovarian follicles through proteome profiling analysis. CD-1 female mice (60 days old) were pipet fed tocopherol stripped corn oil (vehicle control) only or a phthalate mixture (52% DBP, 36% DEHP, and 12% BBP dissolved in vehicle) which modeled human follicular fluid concentrations. The mice were treated with 32µg/kg/day (PHT Mix 32; cumulative estimate in general population) and 500µg/kg/day (PHT Mix 500; cumulative estimate in occupationally exposed individuals) for 10 consecutive days. Proteome profiling of antral follicles (>250µm) was performed via label-free tandem mass spectrometry. A total of 5,417 antral follicle proteins were identified in the three groups, of which 194 were differentially abundant between the vehicle and PHT Mix 32 group, and 136 between the vehicle and PHT Mix 500 group. Gene ontology analysis revealed that the two treatments of the phthalate mixture upregulate and downregulate distinctive processes, supporting non-monotonic effects of phthalates on the antral follicle proteome. Taken together, these results reveal that a human relevant mixture of DBP, BBP, and DEHP alters the antral follicle proteome and merits further evaluation to elucidate the molecular mechanisms by which phthalates cause negative reproductive outcomes.

Dataset Information

Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation.

Dataset's files

Publications

Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation.

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