Proteomics

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Tick (Ixodes ricinus) salivary antigens by LC-MS/MS

ABSTRACT: Here, we challenged rabbits with repeated feeding of Ixodes ricinus adults and observed the formation of specific antibodies against several tick salivary proteins. To identify the salivary antigens, isolated immunoglobulins from repeatedly infested rabbits were utilised for a pull-down from the saliva of pilocarpine-treated ticks. Eluted antigens were separated on 1D SDS-PAGE and analysed by peptide mass fingerprinting. To increase the authenticity of immunogens identified, we also performed, for the first time, de novo assembly of the sialome from I. ricinus females fed for six days, a timepoint used for pilocarpine-salivation.

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Ixodes Ricinus Homo Sapiens (human)

TISSUE(S): Saliva

SUBMITTER: Per Haberkant  

LAB HEAD: Jan Perner

PROVIDER: PXD021370 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

The Central Role of Salivary Metalloproteases in Host Acquired Resistance to Tick Feeding.

Perner Jan J   Helm Dominic D   Haberkant Per P   Hatalova Tereza T   Kropackova Sara S   Ribeiro Jose M JM   Kopacek Petr P  

Frontiers in cellular and infection microbiology 20201118

During feeding on vertebrate hosts, ticks secrete saliva composed of a rich cocktail of bioactive molecules modulating host immune responses. Although most of the proteinaceous fraction of tick saliva is of little immunogenicity, repeated feeding of ticks on mammalian hosts may lead to impairment of tick feeding, preventing full engorgement. Here, we challenged rabbits with repeated feeding of both <i>Ixodes ricinus</i> nymphs and adults and observed the formation of specific antibodies against  ...[more]

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