Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Homo Sapiens (human) Betainfluenzavirus Alphainfluenzavirus
TISSUE(S): Blood
SUBMITTER: Andrew McArdle
LAB HEAD: Michael Levin
PROVIDER: PXD021575 | Pride | 2021-01-04
REPOSITORIES: Pride
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PloS one 20201223 12
A novel approach to recover and identify immune complexes (ICs) was developed using size exclusion chromatography (SEC) and affinity chromatography on immunoglobulin binding columns (HiTrap Protein G). The purification process was monitored by 1D SDS-PAGE, protein staining, Western blotting and, finally, liquid chromatography tandem mass spectrometry (LC MS/MS) was used to identify the recovered antigens. This approach was applied to serum with artificially created immune complexes (ICs) compris ...[more]