Dataset Information
Mitigating in-column artificial modification in HTLC for bottom-up proteomics and quality control of biopharmaceuticals
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human) Francisella Tularensis Subsp. Holarctica (strain Lvs)
SUBMITTER: 
Juraj Lenco
LAB HEAD: Dr Juraj
PROVIDER: PXD052716 | Pride | 2024-09-11
REPOSITORIES: Pride
Dataset's files
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Publications
Mitigating In-Column Artificial Modifications in High-Temperature LC-MS for Bottom-Up Proteomics and Quality Control of Protein Biopharmaceuticals.
Analytical chemistry 20240828 36
Elevating the column temperature is an effective strategy for improving the chromatographic separation of peptides. However, high temperatures induce artificial modifications that compromise the quality of the peptide analysis. Here, we present a novel high-temperature LC-MS method that retains the benefits of a high column temperature while significantly reducing peptide modification and degradation during reversed-phase liquid chromatography. Our approach leverages a short inline trap column m ...[more]
