Dataset Information

Identification of human GRHL1 interactors and post-translational modifications by LC-MS/MS

ABSTRACT: Aim of the project was to identify interactors and post-translational modifications of recombinant 3xFLAG-6xHis-tagged human GRHL1, tandem-affinity purified from isolated HEK293 nuclei treated with DMSO as control or with the phytochemical papaverine.

INSTRUMENT(S): nanoACQUITY UPLC, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Fabian Fischer

LAB HEAD: Michael Ristow

PROVIDER: PXD021768 | Pride | 2021-11-02

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20181121_11493_scaffold_swiss_human_D1_PH1.sf3	Other
	20181121ff_11493_Ctrl_D1.mgf	Mgf
	20181121ff_11493_Ctrl_D1.raw	Raw
	20181121ff_11493_Ctrl_PH1.mgf	Mgf
	20181121ff_11493_Ctrl_PH1.raw	Raw

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Publications

Grainyhead 1 acts as a drug-inducible conserved transcriptional regulator linked to insulin signaling and lifespan.

Grigolon Giovanna G Araldi Elisa E Erni Reto R Wu Jia Yee JY Thomas Carolin C La Fortezza Marco M Laube Beate B Pöhlmann Doris D Stoffel Markus M Zarse Kim K Carreira Erick M EM Ristow Michael M Fischer Fabian F

Nature communications 20220110 1

Aging is impacted by interventions across species, often converging on metabolic pathways. Transcription factors regulate longevity yet approaches for their pharmacological modulation to exert geroprotection remain sparse. We show that increased expression of the transcription factor Grainyhead 1 (GRH-1) promotes lifespan and pathogen resistance in Caenorhabditis elegans. A compound screen identifies FDA-approved drugs able to activate human GRHL1 and promote nematodal GRH-1-dependent longevity. ...[more]

PMID: 35013237

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