Dataset Information

Identification of N-linked glycosylated peptides in the open ocean diatom Thalassiosira oceanica

ABSTRACT: N-linked glycosylation is a posttranslational modification affecting protein folding and protein function. The N-linked glycosylation pathway in algae is poorly characterized, and further knowledge is needed to understand the cell biology of algae and the evolution of N-linked glycosylation. This study investigated the N-linked glycosylation pathway in the open ocean diatom Thalassiosira oceanica and identified N-linked glycosylated peptides through solid-phase extraction of N-linked glycosylated peptides (SPEG). We characterized 118 N-linked glycosylated peptides from samples of cells grown in high- and low-iron conditions. The identified peptides had 81% NXT-type motifs, with X being any amino acids except proline. The presence of N-linked glycosylation sites in the iron starvation-induced protein 1a (ISIP1a) confirmed its predicted topology, contributing to the biochemical characterization of ISIP1 proteins.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Thalassiosira Oceanica

TISSUE(S): Cell Suspension Culture

SUBMITTER: Alejandro Cohen

LAB HEAD: Julie LaRoche

PROVIDER: PXD021782 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	150729_0583_073_S01_CID-2.msf	Msf
	150729_0583_073_S01_CID-2.msfView	Msf
	150729_0583_073_S01_CID-2.pdResult	Other
	150729_0583_073_S01_CID-2.pdResultView	Other
	150729_0583_073_S01_CID.raw	Raw

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Publications

N-linked glycosylation enzymes in the diatom Thalassiosira oceanica exhibit a diel cycle in transcript abundance and favor for NXT-type sites.

Behnke Joerg J Cohen Alejandro M AM LaRoche Julie J

Scientific reports 20210205 1

N-linked glycosylation is a posttranslational modification affecting protein folding and function. The N-linked glycosylation pathway in algae is poorly characterized, and further knowledge is needed to understand the cell biology of algae and the evolution of N-linked glycosylation. This study investigated the N-linked glycosylation pathway in Thalassiosira oceanica, an open ocean diatom adapted to survive at growth-limiting iron concentrations. Here we identified and annotated the genes coding ...[more]

PMID: 33547363

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