Dataset Information

N-glycosite mapping in Brugia malayi female worms, male worms, and microfilariae

ABSTRACT: N-linked glycosylation is a critical post translational modification of eukaryotic proteins. N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions. Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite. In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS. We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae. Fbs1 enrichment of N-glycosylated peptides enhanced the identification of N-glycosites. Our data identified 582 N-linked glycoproteins with 1273 N-glycosites. Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins. Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level. These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Brugia Malayi

TISSUE(S): Whole Body

SUBMITTER: Fana Mersha

LAB HEAD: Jeremy M. Foster

PROVIDER: PXD039002 | Pride | 2023-04-23

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Bm_male_Total_PNG_3ug_3hr_Top10_LM_n1200_QE_Aur_Son_022520_Run2.raw	Raw
	Bm_male_Total_tryp_PNG_3ug_3hr_Top10_LM_n1200_QE_Aur_Son_022520_Run1.raw	Raw
	Bm_male_Total_tryp_PNG_3ug_3hr_Top10_LM_n1200_QE_Aur_Son_022520_Run3.raw	Raw
	Bm_male_pre-enrich_control_tryp_PNG_3ug_...op10_LM_n1200_QE_Aur_Son_022520_Run1.mgf	Mgf
	Bm_male_pre-enrich_control_tryp_PNG_3ug_...op10_LM_n1200_QE_Aur_Son_022520_Run1.mzid.gz	Mzid

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N-glycosite mapping in Brugia malayi female worms, male worms, and microfilariae

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