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Function and Regulation of the pyruvate transporter CstA in Escherichia coli

ABSTRACT: Pyruvate is a central metabolite that connects many metabolic pathways in living organisms. To meet the cellular pyruvate requirements, Escherichia coli most likely has three pyruvate uptake systems, BtsT, YhjX and CstA. Transport studies in intact cells proved that CstA is a high specific pyruvate transporter with moderate affinity, which is energized by a proton gradient. When the cells of a reporter strain were cultured in complex medium, cstA expression was bimodal, with the maximum being reached in the stationary phase. A DNA affinity capture assay combined with mass spectrometry and an in vivo reporter assay identified Fis as repressor in addition to the known cAMP-CRP as activator for cstA expression.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Escherichia Coli

SUBMITTER: Ignasi Forne

LAB HEAD: Prof. Dr. Kirsten Jung

PROVIDER: PXD021798 | Pride | 2020-12-02

REPOSITORIES: Pride

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			Action	DRS
	Ref3094_AG_01_20200506.raw	Raw
	Ref3094_AG_02_20200506.raw	Raw
	Ref3094_AG_03_20200506.raw	Raw
	Ref3094_AG_04_20200506.raw	Raw
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