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Proteomics of Human Milk: Definition of a Discovery Workflow for Clinical Research Studies

ABSTRACT: Proteins in milk have been studied for decades and proteomics, peptidomics, and glycoproteomics are the main approaches previously deployed to decipher the proteome of human milk. In the present work, we aimed at implementing a highly automated pipeline for the proteomic analysis of human milk with liquid chromatography (LC) mass spectrometry (MS). Commercial human milk samples were used to evaluate and optimize workflows.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Milk

SUBMITTER: Loïc Dayon

LAB HEAD: Loïc Dayon

PROVIDER: PXD021974 | Pride | 2021-08-01

REPOSITORIES: Pride

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			Action	DRS
	HM_Replication_TMT_Exp1_01.raw	Raw
	HM_Replication_TMT_Exp1_01.rawF089374.mzid.gz	Mzid
	HM_Replication_TMT_Exp1_01.rawF089374.mzid_HM_Replication_TMT_Exp1_01.raw_F089374.MGF	Mzid
	HM_Replication_TMT_Exp1_02.raw	Raw
	HM_Replication_TMT_Exp1_02.rawF089376.mzid.gz	Mzid

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Publications

Proteomics of Human Milk: Definition of a Discovery Workflow for Clinical Research Studies.

Dayon Loïc L Macron Charlotte C Lahrichi Sabine S Núñez Galindo Antonio A Affolter Michael M

Journal of proteome research 20210326 5

Milk is a complex biological fluid composed mainly of water, carbohydrates, lipids, proteins, and diverse bioactive factors. Human milk represents a unique tailored source of nutrients that adapts during lactation to the specific needs of the developing infant. Proteins in milk have been studied for decades, and proteomics, peptidomics, and glycoproteomics are the main approaches previously deployed to decipher the proteome of human milk. In the present work, we aimed at implementing a highly au ...[more]

PMID: 33769819

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