Dataset Information

Proteomics studies of outer and inner cellular layers of tomato root-tips for Al stress

ABSTRACT: Excess levels of Al3+ become highly harmful to plant roots. The epidermal and outer cortical layers of root-tips experience more severe damages than the inner tissues from Al toxicity. This project used laser capture microdissection (LCM) technology to isolate the outer and inner cell layers of cells in root-tips. Tomato "MicroTom" plants were grown in hydroponic solution supplemented with Al. Seeds derived from plants previously exposed to four generations of Al-treated solutions were considered stress-acclimated, these seeds were named G4Al+. Seeds harvested from soil-grown plants were also used, these seeds were named G0 seeds. Tandem mass tag (TMT) proteomics was used to identify differential proteomics responses among cells localized at different spaces in root-tips and those between the stress-acclimated and non-acclimated plants.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Plant Cell, Root

SUBMITTER: Ted Thannhauser

LAB HEAD: Theodore Thannhauser <tt34@cornell.edu>

PROVIDER: PXD021994 | Pride | 2022-02-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	5July2018_Suping_10plexTMT_Exp1.msf	Msf
	5July2018_Suping_10plexTMT_Exp1_F1.raw	Raw
	5July2018_Suping_10plexTMT_Exp1_F10.raw	Raw
	5July2018_Suping_10plexTMT_Exp1_F11.raw	Raw
	5July2018_Suping_10plexTMT_Exp1_F12.raw	Raw

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Publications

Using single cell type proteomics to identify Al-induced proteomes in outer layer cells and interior tissues in the apical meristem/cell division regions of tomato root-tips.

Potts Jesse J Li Hui H Qin YaoGuo Y Wu Xingbo X Hui Dafeng D Nasr Kamal Al KA Zhou Suping S Yong Yang Y Fish Tara T Liu Jiping J Thannhauser Theodore W TW

Journal of proteomics 20220120

Aluminum (Al) toxicity primarily targets the root tips, inhibiting root growth and function and leading to crop yield losses on acidic soils. Previously we reported using laser capture microdissection (LCM) proteomics to identify Al-induced proteins in the outer layer cells in the transitional zone of tomato root-tips. This study aims to further characterize Al-induced proteomic dynamics from the outer to interior tissues, thus providing a panoramic view reflecting Al resistance in the root tip ...[more]

PMID: 35066208

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