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Proteomic analysis of Histoplasma capsulatum under zinc deprivation

ABSTRACT: The project aimmed to characterize the proteomic response of the human pathogenic fungus Histoplasma capsulatum to zinc poor environment. For that yeast cells were incubated in two conditions: zinc replete (with 20uM fo zinc sulfate) and zinc depleted (suppplemented with diethylenetriaminepentaacetic acid. Finaly teh proteins regulaed by zinc availability were identified.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Histoplasma Capsulatum G186ar

TISSUE(S): Fungal Cell

DISEASE(S): Histoplasmosis

SUBMITTER: Alexandre Bailao

LAB HEAD: Alexandre Melo Bailao

PROVIDER: PXD022039 | Pride | 2020-10-21

REPOSITORIES: Pride

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Project description:Paracoccidioidomycosis (PCM) is an endemic mycosis in Latin America. The PCM is a systemic disease caused by the thermo dimorphic fungus Paracoccidioides spp, which can exist as mycelia and yeast forms. The mycelia form can be found in environment at temperatures between 18 °C and 25 °C and produces spores or conidia. The PCM infection route occurs by the inhalation of conidia. Once in the lungs, these particles are converted into yeasts pathogenic form. Until now, metabolic aspects and virulence factors related to Paracoccidioides lutzii infective propagule are little understood. In this concern, a global proteomic study of P. lutzii conidia was performed. For conidia production, the mycelia of the isolate Pb01 were cultured in potato agar medium during 90 days at 18 °C. Obtained conidia were collected and purified. The proteins were extracted and subjected to tryptic digestion followed by identification by NanoUPLC-MSE approach. We identified a total of 242 proteins in P. lutzii conidia which were subjected to in silico functional analysis Proteins putatively acting as adhesins, such as GAPDH, enolase, and the glycoprotein β-1,3-glucanosyltransferase (gel2) were identified during our analysis. We also identified proteins related to signal transduction pathways, such as Ras and RhoA GTPases, previously demonstrated to be required for morphologic changes in pathogenic fungi. Proteins related to evasion, defense and virulence were also identified. Proteins acting during temperature shifts or oxidative stress provided by the host environment such as HSP90, catalase, mitochondrial peroxiredoxin Prx1 were identified. It were also identified proteins related to energy production and protein biosynthesis, which can provide important aspects to survival of this resting cells. These results highlight that P. lutzii conidia contain proteins that can contribute to its maintenance in the environment and also molecules related to host-pathogen interactions.

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Proteomic analysis of Histoplasma capsulatum under zinc deprivation

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