Proteomics

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Proteomic characterization of synaptosomes from human substantia nigra indicates altered mitochondrial translation in Parkinson’s disease


ABSTRACT: Parkinson´s disease (PD) feature a progressive degeneration of dopaminergic neurons in the substantia nigra (SN). Additionally, numerous studies indicate an altered synaptic function during the course of PD. In order to characterize the proteome of synaptosomes isolated from the substantia nigra and to gain new insights into the molecular processes underlying the alteration of synaptic function in PD, a proteomic study was conducted. Synaptosomes were isolated from substantia nigra tissue of control subjects free of pathology (N = 5) and individuals at advanced PD stages (N = 5) by density gradient centrifugation and further analyzed by mass spectrometry. 362 proteins were identified and assigned to the synaptosomal core proteome. This core proteome includes all proteins expressed within the synapses without regards to data analysis software, gender, age or disease. The CD9 antigen was overrepresented and fourteen proteins, among them Thymidine kinase 2 (TK2), mitochondrial, 39S ribosomal protein L37, mitochondrial,, Neurolysin, and Methionine-tRNA ligase, mitochondrial (MARS2)) were underrepresented suggesting an alteration in mitochondrial translation in PD synaptosomes.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Neuron

DISEASE(S): Parkinson's Disease

SUBMITTER: Britta Eggers  

LAB HEAD: Prof. Dr. Katrin Marcus

PROVIDER: PXD022092 | Pride | 2021-06-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Annotation.xlsx Xlsx
F065935.dat Other
F065935.mzid.gz Mzid
F065936.dat Other
F065936.mzid.gz Mzid
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Publications

Proteomic Characterization of Synaptosomes from Human Substantia Nigra Indicates Altered Mitochondrial Translation in Parkinson's Disease.

Plum Sarah S   Eggers Britta B   Helling Stefan S   Stepath Markus M   Theiss Carsten C   Leite Renata E P REP   Molina Mariana M   Grinberg Lea T LT   Riederer Peter P   Gerlach Manfred M   May Caroline C   Marcus Katrin K  

Cells 20201202 12


The pathological hallmark of Parkinson's disease (PD) is the loss of neuromelanin-containing dopaminergic neurons within the substantia nigra pars compacta (SNpc). Additionally, numerous studies indicate an altered synaptic function during disease progression. To gain new insights into the molecular processes underlying the alteration of synaptic function in PD, a proteomic study was performed. Therefore, synaptosomes were isolated by density gradient centrifugation from SNpc tissue of individua  ...[more]

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