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C57BL/6J mice B and T cell LC-MSMS study

ABSTRACT: Proteome isolated from C57BL/6J mouse B and T cells are evluated for the presence of novel open reading frame products (nORFs). Translation products encoded by non canonical or novel open reading frame (ORF) genomic regions are generally considered too small to play any significant biological role, and dismissed as inconsequential. We conduct a systematic study of novel ORFs to gain new insights into normal biological and disease processes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, B Cell, T Cell

SUBMITTER: Chaitanya Erady

LAB HEAD: Dr. Sudhakaran Prabakaran

PROVIDER: PXD022099 | Pride | 2021-09-09

REPOSITORIES: Pride

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Pan-cancer analysis of transcripts encoding novel open-reading frames (nORFs) and their potential biological functions.

Erady Chaitanya C Boxall Adam A Puntambekar Shraddha S Suhas Jagannathan N N Chauhan Ruchi R Chong David D Meena Narendra N Kulkarni Apurv A Kasabe Bhagyashri B Prathivadi Bhayankaram Kethaki K Umrania Yagnesh Y Andreani Adam A Nel Jean J Wayland Matthew T MT Pina Cristina C Lilley Kathryn S KS Prabakaran Sudhakaran S

NPJ genomic medicine 20210125 1

Uncharacterized and unannotated open-reading frames, which we refer to as novel open reading frames (nORFs), may sometimes encode peptides that remain unexplored for novel therapeutic opportunities. To our knowledge, no systematic identification and characterization of transcripts encoding nORFs or their translation products in cancer, or in any other physiological process has been performed. We use our curated nORFs database (nORFs.org), together with RNA-Seq data from The Cancer Genome Atlas ( ...[more]

PMID: 33495453

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